Minami Kazuhiko, Kogashiwa Yasunao, Ebihara Yasuhiro, Nakahira Mitsuhiko, Sugasawa Masashi, Fujino Takashi, Yasuda Masanori
a Department of Head and Neck Surgery , Saitama Medical University International Medical Center , Hidaka , Saitama , Japan.
b Department of Pathology , Saitama Medical University International Medical Center , Hidaka , Saitama , Japan.
Acta Otolaryngol. 2017 Oct;137(10):1121-1126. doi: 10.1080/00016489.2017.1339327. Epub 2017 Jul 2.
The objectives were to determine the prevalence of human papillomavirus (HPV) in mobile tongue cancer (MTC) and evaluate associations and survival.
Patients who underwent surgical resection as primary treatment for MTC (n = 127) were retrospectively evaluated. Formalin-fixed paraffin-embedded (FFPE) specimens were assessed for p16 and p53 by immunohistochemistry; for HPV DNA by nested multiplex polymerase chain reaction (PCR) using two pairs of consensus primers (MY09-MY11 and GP5+-GP6+); and for E6 and E7 oncogenes from 13 high-risk HPV types (16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66, and 68) by real-time reverse transcription PCR (RT-PCR).
There were 18 (14.2%) p16-positive, 45 (35.4%) p53-positive, 9 (7.1%) HPV DNA-positive, and 7 (5.5%) E6 and/or E7 mRNA-positive tumors, but the correlation of all pairs was poor. There was no demographic or histopathologic association with HPV status. Cause-specific survival was significantly better with p16-positive than with p16-negative tumors (p = .037).
The prevalence of HPV and p16 positivity was relatively low and p16 status was a poor surrogate marker for HPV status. The results showed the importance of p16 expression in prognosticating mobile tongue cancer.
本研究旨在确定人乳头瘤病毒（HPV）在移动性舌癌（MTC）中的流行率，并评估其相关性及生存率。
对127例行手术切除作为MTC主要治疗方法的患者进行回顾性评估。采用免疫组化法检测福尔马林固定石蜡包埋（FFPE）标本中的p16和p53；使用两对共有引物（MY09-MY11和GP5+-GP6+）通过巢式多重聚合酶链反应（PCR）检测HPV DNA；采用实时逆转录PCR（RT-PCR）检测13种高危HPV类型（16、18、31、33、35、39、45、52、56、58、59、66和68）的E6和E7癌基因。
18例（14.2%）肿瘤p16阳性，45例（35.4%）p53阳性，9例（7.1%）HPV DNA阳性，7例（5.5%）E6和/或E7 mRNA阳性，但所有配对之间的相关性较差。HPV状态与人口统计学或组织病理学无关联。p16阳性肿瘤的特定病因生存率显著高于p16阴性肿瘤（p = 0.037）。
HPV和p16阳性率相对较低，p16状态不是HPV状态的良好替代标志物。结果显示了p16表达在移动性舌癌预后评估中的重要性。
