患有舌鳞状细胞癌的年轻成年患者在无人类乳头瘤病毒感染的情况下强烈表达p16。
Young adult patients with squamous cell carcinoma of the tongue strongly express p16 without human papillomavirus infection.
作者信息
Tachibana Tomoyasu, Orita Yorihisa, Gion Yuka, Miki Kentaro, Ikegami Kana, Marunaka Hidenori, Makino Takuma, Akagi Yusuke, Akisada Naoki, Tsumura Munechika, Ito Toshihiro, Yoshino Tadashi, Nishizaki Kazunori, Sato Yasuharu
机构信息
a Department of Otolaryngology , Japanese Red Cross Society Himeji Hospital , Himeji , Japan.
b Department of Otolaryngology, Head and Neck Surgery , Kumamoto University Graduate School of Medicine , Kumamoto , Japan.
出版信息
Acta Otolaryngol. 2019 Jan;139(1):80-84. doi: 10.1080/00016489.2018.1541506. Epub 2019 Feb 3.
BACKGROUND
Long-term smoking and drinking are known to contribute to the onset of tongue cancer (TC). However, the increasing incidence of TC in younger adults has been suggested to be associated with other factors.
OBJECTIVES
The present study investigated the relationship between TC and human papillomavirus (HPV) infection.
MATERIAL AND METHODS
Clinical records and surgically resected specimens from 86 patients (<40-years-old, n = 12; ≥40-years-old, n = 74) with TC were analyzed. Strong nuclear and cytoplasmic p16 staining was considered positive. HPV DNA (high-risk subtypes: 16, 18, 31, 33, 35, 52b, and 58; low-risk subtypes: 6 and 11) was detected using consensus primer-mediated polymerase chain reaction.
RESULTS
Strong p16 expression was observed in 10 (11.6%) patients. HPV DNA was detected in 9 (10.5%) patients (high-risk subtypes, n = 2; low-risk subtypes, n = 7). Strong p16 expression was observed more frequently among younger adults than among older adults (33.3% vs. 8.1%; p = .045). p16 staining did not correlate with the detection of HPV DNA (correlation coefficient, 0.113; p = .300).
CONCLUSIONS AND SIGNIFICANCE
In TC, p16 expression was not associated with HPV infection, suggesting that it may be caused by a different mechanism.
背景
长期吸烟和饮酒已知会促使舌癌（TC）发病。然而，有研究表明，年轻成年人中舌癌发病率的上升与其他因素有关。
目的
本研究调查舌癌与人类乳头瘤病毒（HPV）感染之间的关系。
材料与方法
分析了86例舌癌患者（年龄<40岁，n = 12；年龄≥40岁，n = 74）的临床记录和手术切除标本。细胞核和细胞质p16强染色被视为阳性。采用共识引物介导的聚合酶链反应检测HPV DNA（高危亚型：16、18、31、33、35、52b和58；低危亚型：6和11）。
结果
10例（11.6%）患者观察到p16强表达。9例（10.5%）患者检测到HPV DNA（高危亚型，n = 2；低危亚型，n = 7）。年轻成年人中p16强表达的观察频率高于年长者（33.3%对8.1%；p = 0.045）。p16染色与HPV DNA检测无相关性（相关系数，0.113；p = 0.300）。
结论与意义
在舌癌中，p16表达与HPV感染无关，提示其可能由不同机制引起。
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