Ullah Munzer, Weng Cai-Hong, Li Hui, Sun Shi-Wei, Zhang Hong, Song Ai-Huan, Zhu Hu
a Centre for Bioengineering and Biotechnology , China University of Petroleum (East China) , Qingdao , People's Republic of China.
b Marine Biology Institute of Shandong Province , Qingdao , People's Republic of China.
Environ Technol. 2018 Aug;39(16):2056-2061. doi: 10.1080/09593330.2017.1349189. Epub 2017 Jul 13.
In this study, polyvinyl alcohol (PVA)-degrading bacteria were screened from oil sludge using PVA as a sole source of carbon in the culture medium. A novel strain, SA21, was obtained and identified as a member of the Stenotrophomonas genus based on the analysis of a partial 16S rDNA nucleotide sequence, morphological and biochemical characteristics, and phylogenetic analysis. This Stenotrophomonas isolate had not previously been reported as a PVA-degrading bacterium. Stenotrophomonas sp. strain SA21 degraded 90% of the PVA present in the culture medium after 4 days. The effect of nitrogen sources on the production of PVA-degrading enzyme involved in the biodegradation process was significant, and the enzymatic activity reached 82 U/ml when ammonium nitrate or urea was used in the optimized medium. The information obtained in this study will provide a foundation for improving industrial wastewater treatment.
DCW: dry cell weight; FTIR: Fourier Transform Infrared Spectroscopy; NCBI: National Center for Biotechnology Information; PCR: polymerase chain reaction; PVA: polyvinyl alcohol; SEM: scanning electron microscope.
在本研究中，以聚乙烯醇（PVA）作为培养基中的唯一碳源，从油泥中筛选出PVA降解菌。通过对部分16S rDNA核苷酸序列、形态和生化特征以及系统发育分析，获得了一株新菌株SA21，并鉴定为嗜麦芽窄食单胞菌属的成员。此前尚未报道过这种嗜麦芽窄食单胞菌分离株可作为PVA降解菌。嗜麦芽窄食单胞菌SA21菌株在4天后降解了培养基中90%的PVA。氮源对生物降解过程中PVA降解酶产生的影响显著，在优化培养基中使用硝酸铵或尿素时，酶活性达到82 U/ml。本研究获得的信息将为改善工业废水处理提供基础。
DCW：干细胞重量；FTIR：傅里叶变换红外光谱；NCBI：美国国家生物技术信息中心；PCR：聚合酶链反应；PVA：聚乙烯醇；SEM：扫描电子显微镜。
