School of Biology, Biological Engineering, South China University of Technology, Guangzhou, China.
J Basic Microbiol. 2019 Apr;59(4):368-374. doi: 10.1002/jobm.201800468. Epub 2019 Jan 29.
To discuss the microbial structure that develops during the course of compost burials of polyvinyl alcohol (PVA)-based material and analyze the effect of the degree of PVA alcoholysis on the PVA-degrading ability of each strain. Two bacterial strains exhibiting a high level of PVA-degrading ability and two mixed cultures were isolated from compost loaded with PVA-based material. The rod-shaped Gram-positive bacterium DG22 was identified by 16S rDNA sequencing analysis as Bacillus sp. DG22, and the rod-shaped Gram-negative bacterium DG14 was identified as Paenibacillus sp. DG14. Biodegradation rates were determined by iodometric analysis within 8 days of culturing on selective media. The biodegradation rates for PVA1799 by DG22, DG14, T1 (mixed culture from a 36-month burial), and T2 (mixed culture from a 24-month burial) were 54.47, 46.59, 69.62, and 79.04%, respectively, and those of PVA1788 were 74.99, 67.27, 54.43, and 50.76%, respectively. DG22 and DG14 are the first known Bacillus sp. and Paenibacillus sp. able to utilize PVA solely. However, the higher degree of PVA alcoholysis (AD) had a negative effect on the biodegradation of PVA.
讨论聚乙烯醇（PVA）基材料在堆肥埋葬过程中形成的微生物结构，并分析 PVA 醇解度对各菌株 PVA 降解能力的影响。从装有 PVA 基材料的堆肥中分离出两株具有高 PVA 降解能力的细菌菌株和两种混合培养物。通过 16S rDNA 测序分析，棒状革兰氏阳性菌 DG22 被鉴定为芽孢杆菌 DG22，棒状革兰氏阴性菌 DG14 被鉴定为巨大芽孢杆菌 DG14。在选择性培养基上培养 8 天后，通过碘量法分析确定生物降解率。DG22、DG14、T1（来自 36 个月埋葬的混合培养物）和 T2（来自 24 个月埋葬的混合培养物）对 PVA1799 的生物降解率分别为 54.47%、46.59%、69.62%和 79.04%，对 PVA1788 的生物降解率分别为 74.99%、67.27%、54.43%和 50.76%。DG22 和 DG14 是已知的第一株能够单独利用 PVA 的芽孢杆菌和巨大芽孢杆菌。然而，较高的 PVA 醇解度（AD）对 PVA 的生物降解有负面影响。
