来自油泥样品的RA23对聚乙烯醇的降解作用。
Polyvinyl alcohol degradation by RA23 from oil sludge sample.
作者信息
Ullah Munzer, Li Hui, Sun Shi-Wei, Weng Cai-Hong, Zhang Hong, Zhu Hu
机构信息
1Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), 66 Changjiang West Road, Qingdao, 266580 People's Republic of China.
2Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial University Engineering Research Center of Industrial Biocatalysis, College of Chemistry and Materials Science, Fujian Normal University, 32 Shangsan Road, Fuzhou, 350007 People's Republic of China.
出版信息
3 Biotech. 2019 Oct;9(10):350. doi: 10.1007/s13205-019-1882-6. Epub 2019 Sep 3.
A novel polyvinyl alcohol (PVA)-degrading strain RA23 was isolated from an oil sludge sample and environmental factors affecting its PVA degradation efficiency were optimized in detail. Inorganic nitrogen source, ammonium chloride (NHCl), was found to be the best nitrogen source and enhanced the PVA degradation rate greatly. The optimal medium for PVA biodegradation consisted of (g/L) PVA 1, NHCl 1, KHPO 1.6, MgSO·7HO 0.05, FeSO·6HO 0.02, CaCl 0.05, NaCl 0.02. The optimal temperature and pH for PVA biodegradation by strain RA23 was 28 °C and 7.0, respectively, and 85% of 0.1% PVA was degraded after 5 days under these conditions. FTIR studies showed that the carboxylic acids (possibly including aldehyde or ketone) could be the intermediate product of PVA biodegradation. The investigation of strain RA23 for PVA degradation will provide important information to facilitate the removal of wastewater pollution in industrial zones.
从油泥样品中分离出一种新型聚乙烯醇（PVA）降解菌株RA23，并详细优化了影响其PVA降解效率的环境因素。发现无机氮源氯化铵（NH₄Cl）是最佳氮源，能极大提高PVA降解率。PVA生物降解的最佳培养基组成为（g/L）：PVA 1、NH₄Cl 1、KH₂PO₄ 1.6、MgSO₄·7H₂O 0.05、FeSO₄·6H₂O 0.02、CaCl₂ 0.05、NaCl 0.02。菌株RA23进行PVA生物降解的最佳温度和pH分别为28℃和7.0，在此条件下，5天后0.1%的PVA有85%被降解。傅里叶变换红外光谱（FTIR）研究表明，羧酸（可能包括醛或酮）可能是PVA生物降解的中间产物。对菌株RA23进行PVA降解的研究将为促进工业区废水污染的去除提供重要信息。
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