Institute for Chemical and Thermal Process Engineering, Technische Universität Braunschweig, Germany; Center of Pharmaceutical Engineering, Technische Universität Braunschweig, Germany.
Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Germany; Center of Pharmaceutical Engineering, Technische Universität Braunschweig, Germany.
Eur J Pharm Biopharm. 2018 May;126:104-107. doi: 10.1016/j.ejpb.2017.06.026. Epub 2017 Jun 29.
Hyperforin is a major metabolite of the medicinal plant Hypericum perforatum (St. John's Wort) and has recently been found in hormone induced root cultures. The objective of this study is to identify a downstream process for the production of a hyperforin-rich extract with maximum extraction efficiency and minimal decomposition. The maximum extraction time was found to be 60min. The comparison of two equipment concepts for the extraction and solvent evaporation was performed employing two different solvents. While the rotary mixer showed better results for the extraction efficiency than a stirred vessel, the latter set-up was able to handle larger volumes but did not meet all process requirements. For the evaporation the prompt evaporation of the extraction agent using nitrogen stripping led to minor decomposition. In a 5L stirred vessel, the highest specific extraction of hyperforin was 4.3mg hyperforin/g dry weight bio material. Parameters for the equipment design for extraction and solvent evaporation were determined based on the experimental data.
金丝桃素是药用植物贯叶连翘（圣约翰草）的主要代谢产物，最近在激素诱导的根培养物中也有发现。本研究的目的是确定一种生产富含金丝桃素提取物的下游工艺，以达到最高提取效率和最小分解。最大提取时间为 60min。通过使用两种不同的溶剂，对两种提取和溶剂蒸发设备概念进行了比较。虽然旋转混合器在提取效率方面的表现优于搅拌釜，但后者能够处理更大的体积，但不符合所有工艺要求。对于蒸发，使用氮气吹扫快速蒸发萃取剂会导致少量分解。在 5L 搅拌釜中，金丝桃素的最高比提取量为 4.3mg 金丝桃素/g 干重生物材料。根据实验数据确定了提取和溶剂蒸发设备设计的参数。
