Liu Yu-Fan, Huang Sha, Yao Bin, Li Zhao, Li Xiang, Fu Xiao-Bing, Wu Xu
1Southern Medical University, Guangzhou 510515, China; 2Department of Thoracic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Jun 20;37(6):761-766. doi: 10.3969/j.issn.1673-4254.2017.06.08.
To evaluate the effect of different microstructures prepared by three-dimensional (3D) bioprinting on proliferation and viability of the murine epithelial stem cells in vitro.
3D cell-laden microstructures were constructed using 3 different printing nozzles with diameters of 210, 340, and 420 µm. Fluorescence microscopy and the live/dead assay kit were used to observe the proliferation and viability of the murine epithelial stem cells in the microstructures.
All the 3D cell-laden micro-structures were capable of promoting the proliferation of murine epithelial stem cells. In the 3 groups of micro-structures, the cell viability decreased significantly with time until 7 days after printing (P<0.01), but at 14 days after the printing, the cell viability increased significantly as compared with that at 7 days (P<0.01). The viability of the cells was significantly higher in the microstructure printed using a 420 µm nozzle than in the microstructures printed with 210 µm and 340 µm nozzles (P<0.01).
The microstructure printed with a 420 µm nozzle can stably promote the proliferation of murine epithelial stem cells and maintain a high level of cell viability, suggesting the feasibility of constructing tissue-engineered epidermis and full-thickness skin graft using 3D bioprinting technique.
评估三维（3D）生物打印制备的不同微观结构对小鼠上皮干细胞体外增殖和活力的影响。
使用3种不同直径（210、340和420 µm）的打印喷嘴构建载有细胞的3D微观结构。采用荧光显微镜和活/死检测试剂盒观察微观结构中小鼠上皮干细胞的增殖和活力。
所有载有细胞的3D微观结构均能促进小鼠上皮干细胞的增殖。在这3组微观结构中，细胞活力随时间显著下降，直至打印后7天（P<0.01），但在打印后14天，细胞活力与7天时相比显著增加（P<0.01）。使用420 µm喷嘴打印的微观结构中的细胞活力显著高于使用210 µm和340 µm喷嘴打印的微观结构（P<0.01）。
用420 µm喷嘴打印的微观结构能稳定促进小鼠上皮干细胞的增殖并维持较高水平的细胞活力，提示使用3D生物打印技术构建组织工程表皮和全层皮肤移植的可行性。
