Magro Daniéla Oliveira, Kotze Paulo Gustavo, Martinez Carlos Augusto Real, Camargo Michel Gardere, Guadagnini Dioze, Calixto Antonio Ramos, Vasques Ana Carolina Junqueira, Ayrizono Maria de Lourdes Setsuko, Geloneze Bruno, Pareja José Carlos, Saad Mario José, Coy Claudio Saddy Rodrigues
Department of Surgery, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil.
Colorectal Surgery Unit, Cajuru University Hospital, Catholic University of Paraná, Curitiba, Brazil.
Intest Res. 2017 Jul;15(3):352-357. doi: 10.5217/ir.2017.15.3.352. Epub 2017 Jun 12.
BACKGROUND/AIMS: Lipopolysaccharide (LPS) is a molecule formed by lipids and polysaccharides and is the major cell wall component of gram-negative bacteria. High LPS levels are known to block CD26 expression by activating Toll-like receptor 4. The aim of this study was to correlate the serum levels of LPS and CD26 in Crohn's disease (CD) patients with serum levels of C-reactive protein (CRP), interleukins, CD activity index, and tumor necrosis factor-α (TNF-α).
Serum samples were collected from 27 individuals (10 with active CD, 10 with inactive CD, and 7 controls) and the levels of LPS, CD26, TNF-α, interleukin-1β (IL-1β), IL-6, IL-17, and CRP were determined by enzyme-linked immunosorbent assay. The levels of LPS and CD26 were then tested for correlation with TNF-α, IL-1β, IL-6, IL-17, and CRP.
Serum levels of LPS were significantly elevated in the active CD group (=0.003). Levels of IL-1β (=0.002), IL-6 (=0.003), and IL-17 (<0.001) were lower in the CD groups. Serum TNF-α levels were increased in the active CD group. The CRP levels were elevated in the CD groups when compared to controls (<0.001). The CD26 levels were lower in the CD groups than in the control group (<0.001). Among the variables analyzed, there was a correlation between LPS and CRP (r=-0.53, =0.016) in the CD groups.
Individuals with CD exhibited higher serum levels of LPS varying from a 2- to 6-fold increase depending on disease activity, when compared with healthy controls. CD26 levels were lower in the CD groups. Both LPS and CD26 correlated with disease severity and serve as potential CD biomarkers.
背景/目的：脂多糖（LPS）是一种由脂质和多糖组成的分子，是革兰氏阴性菌细胞壁的主要成分。已知高LPS水平可通过激活Toll样受体4来阻断CD26表达。本研究的目的是将克罗恩病（CD）患者血清中LPS和CD26的水平与C反应蛋白（CRP）、白细胞介素、CD活动指数及肿瘤坏死因子-α（TNF-α）的血清水平进行关联分析。
收集27名个体（10名活动期CD患者、10名非活动期CD患者和7名对照者）的血清样本，采用酶联免疫吸附测定法测定LPS、CD26、TNF-α、白细胞介素-1β（IL-1β）、IL-6、IL-17和CRP的水平。然后检测LPS和CD26水平与TNF-α、IL-1β、IL-6、IL-17和CRP的相关性。
活动期CD组血清LPS水平显著升高（=0.003）。CD组中IL-1β（=0.002）、IL-6（=0.003）和IL-17（<0.001）水平较低。活动期CD组血清TNF-α水平升高。与对照组相比，CD组CRP水平升高（<0.001）。CD组CD26水平低于对照组（<0.001）。在分析的变量中，CD组LPS与CRP之间存在相关性（r=-0.53，=0.016）。
与健康对照相比，CD患者血清LPS水平较高，根据疾病活动度增加2至6倍。CD组CD26水平较低。LPS和CD26均与疾病严重程度相关，可作为潜在的CD生物标志物。
