Liu Lan, Salam Nimaichand, Jiao Jian-Yu, E Shun-Mei, Chen Cha, Fang Bao-Zhu, Xiao Min, Li Mo, Li Wen-Jun, Qu Ping-Hua
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Department of Laboratory Medical Science, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2178-2183. doi: 10.1099/ijsem.0.001917. Epub 2017 Jul 3.
A taxonomic study was performed on strain SYSU D3-2T, isolated from coastal seawater near the estuary of Pearl River in southern China. The strain was observed to be Gram-reaction-negative, non-motile and non-spore-forming. Cells were found to be of coccobacilli shape. Chemotaxonomic analysis of the plasma membrane revealed ubiquinone-8 as the respiratory quinone, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, an unidentified aminophospholipid and an unidentified phospholipid as the polar lipids, and anteiso-C15 : 0, C18 : 0 and anteiso-C17 : 0 as the major fatty acids (>10 % of total fatty acids). Comparison of 16S rRNA gene sequences showed that strain SYSU D3-2T shared maximum similarities with Caedibacter taeniospiralis 51T (92.3 %) and Fangia hongkongensis UST040201-002T (90.6 %), while sharing 85.8-90.0 % similarity with species of the genera Allofrancisella and Francisella. Phylogenetic dendrograms based on the 16S rRNA gene sequences showed that the strain clustered within the family Francisellaceae, but formed a separate lineage closely linked to Caedibactertaeniospiralis 51T and F. hongkongensis UST040201-002T. Based on the findings of the polyphasic taxonomic study, strain SYSU D3-2T is proposed to be recognized as a representative of a novel species of a new genus within the order Thiotrichales, with the name Cysteiniphilum litorale gen. nov., sp. nov. The type strain of the type species is SYSU D3-2T (=NBRC 112441T=DSM 101832T=KCTC 52386T=CGMCC 1.15758T).
对从中国南方珠江口附近沿海海水中分离得到的菌株SYSU D3-2T进行了分类学研究。观察到该菌株革兰氏反应阴性、无运动性且不形成芽孢。细胞呈球杆菌状。对细胞膜的化学分类分析表明，呼吸醌为泛醌-8，极性脂为二磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基脂、一种未鉴定的氨基磷脂和一种未鉴定的磷脂，主要脂肪酸为anteiso-C15 : 0、C18 : 0和anteiso-C17 : 0（占总脂肪酸的>10 %）。16S rRNA基因序列比较显示，菌株SYSU D3-2T与螺旋带形凯氏杆菌51T（92.3 %）和香港方氏菌UST040201-002T（90.6 %）具有最大相似性，而与嗜弗朗西斯菌属和弗朗西斯菌属的物种相似性为85.8-90.0 %。基于16S rRNA基因序列的系统发育树状图表明，该菌株聚集在弗朗西斯菌科内，但形成了一个与螺旋带形凯氏杆菌51T和香港方氏菌UST040201-002T紧密相连的独立分支。基于多相分类学研究结果，建议将菌株SYSU D3-2T认定为硫发菌目一个新属的新物种的代表，命名为滨海嗜半胱氨酸菌（Cysteiniphilum litorale），新属名，新种名。模式种的模式菌株为SYSU D3-2T（=NBRC 112441T=DSM 101832T=KCTC 52386T=CGMCC 1.15758T）。
