State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
Department of Biological Sciences, Kent State University, Kent, OH, 44242, USA.
Antonie Van Leeuwenhoek. 2019 Sep;112(9):1357-1367. doi: 10.1007/s10482-019-01268-6. Epub 2019 Apr 25.
Strain SYSU M10002 was isolated from a water sample collected from the coastal region of Pearl River estuary, Guangdong Province, southern China. The taxonomic position of the isolate was investigated by polyphasic taxonomic approaches. The isolate was found to be Gram-negative, non-motile, short rods and aerobic. The strain was able to grow at 14-37 °C, pH 6.0-10.0 and in the presence of up to 0.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU M10002 is a member of the family Sphingomonadaceae, with high sequence similarity to Sphingorhabdus buctiana T5 (95.1%). Overall genomic related indices between the genome of strain SYSU M10002 and those of related strains were low to moderate (AAI values < 64.3%; POCP values < 58%), indicating that strain SYSU M10002 represents a novel lineage within the family Sphinogomonadaceae. Strain SYSU M10002 contained homospermidine as its polyamine. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, two unidentified phospholipids and an unidentified aminolipid. Ubiquinone Q-9 (44.9%) and Q-10 (43.2%) were the dominant respiratory quinones, along with a minor amount of Q-8 (11.9%). The predominant cellular fatty acids (> 10%) identified were summed feature 3 (Cω7c and/or Cω6c), summed feature 8 (Cω7c) and C 2-OH. The genomic DNA G+C content was 64.0%. Based on the analyses of the phenotypic, genotypic and phylogenetic characteristics, strain SYSU M10002 is determined to represent a novel species of a novel genus, for which the name Aestuariisphingobium litorale gen. nov., sp. nov. is proposed. The type strain of the species is SYSU M10002 (= KCTC 52944 = NBRC 112961).
SYSU M10002 菌株从中国南部广东省珠江口沿海地区的一个水样中分离得到。通过多相分类方法研究了分离株的分类地位。结果表明，该分离株为革兰氏阴性、非运动、短杆状、需氧菌。该菌株能够在 14-37°C、pH6.0-10.0 和高达 0.5%（w/v）NaCl 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，SYSU M10002 菌株是鞘氨醇单胞菌科的一个成员，与 Sphingorhabdus buctiana T5（95.1%）具有很高的序列相似性。SYSU M10002 菌株基因组与相关菌株之间的全基因组相关指数较低，为 64.3%（AAI 值）；中度相关，为 58%（POCP 值），表明 SYSU M10002 菌株代表了鞘氨醇单胞菌科内的一个新谱系。SYSU M10002 菌株含有同亚精胺作为其多胺。主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、神经酰胺糖脂、两种未鉴定的磷脂和一种未鉴定的胺脂。主要呼吸醌为 ubiquinone Q-9（44.9%）和 Q-10（43.2%），其次是少量的 Q-8（11.9%）。鉴定出的主要细胞脂肪酸（>10%）为 summed feature 3（Cω7c 和/或 Cω6c）、summed feature 8（Cω7c）和 C 2-OH。基因组 DNA G+C 含量为 64.0%。基于表型、基因型和系统发育特征的分析，SYSU M10002 菌株被确定为一个新属的新种，建议将其命名为 Aestuariisphingobium litorale gen. nov.，sp. nov.。该物种的模式菌株为 SYSU M10002（=KCTC 52944=NBRC 112961）。
