Zheng Min-Ling, Jiao Jian-Yu, Dong Lei, Han Ming-Xian, Li Liang-Hui, Xiao Min, Chen Cha, Qu Ping-Hua, Li Wen-Jun
The Second Clinic College, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People's Republic of China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Jun;112(6):877-886. doi: 10.1007/s10482-018-01220-0. Epub 2019 Jan 2.
A Gram-negative, aerobic, non-motile and non-spore forming bacterium, designated strain SYSU WZ-2, was isolated from an estuarine seawater sample. Growth of strain SYSU WZ-2 was observed at temperature range of 10-40° C (optimum, 32 °C), pH range of 6-10 (optimum, pH 7-8) and in the presence of up to 5.0% NaCl (w/v). The DNA G+C content of the novel strain was determined to be 30.1% (genome). The major polar lipids were found to be diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, an unidentified aminolipid, two unidentified aminophospholipids and two unidentified phospholipids. The major fatty acids were C 3-OH (27.5%), Cω9c (19.3%), C (17.0%) and C (12.9%). The respiratory quinone was found to be ubiquinone Q8. Pairwise comparison of the 16S rRNA gene sequence showed that strain SYSU WZ-2 shares high identities with members of the genera Francisella (94.8-95.9%) and Allofrancisella (93.8-94.2%). The phylogenetic dendrograms based on 16S rRNA gene sequences with the members of the family Francisellaceae showed that the strain SYSU WZ-2 formed a distinct phylogenetic lineage well separated from the members of the genera Francisella and Allofrancisella. MALDI-TOF mass spectrometric analysis also depicted a different profile for strain SYSU WZ-2 compared with those of members of the genera Francisella and Allofrancisella. Based on the above results and differences in phenotypic and chemotaxonomic features, strain SYSU WZ-2 is characterized to represent a new species of a novel genus, for which the name Pseudofrancisella aestuarii gen. nov., sp. nov. is proposed (type strain SYSU WZ-2 = KCTC 52557 = CGMCC 1.13718).
从河口海水样本中分离出一株革兰氏阴性、需氧、不运动且不形成芽孢的细菌，命名为SYSU WZ-2菌株。观察到SYSU WZ-2菌株在10-40°C（最适温度为32°C）的温度范围内、pH值6-10（最适pH值7-8）以及存在高达5.0%（w/v）NaCl的条件下生长。该新菌株的DNA G+C含量经测定为30.1%（基因组）。主要极性脂质为双磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、一种未鉴定的氨基脂质、两种未鉴定的氨基磷脂和两种未鉴定的磷脂。主要脂肪酸为C 3-OH（27.5%）、Cω9c（19.3%）、C（17.0%）和C（12.9%）。发现呼吸醌为泛醌Q8。16S rRNA基因序列的成对比较表明，SYSU WZ-2菌株与弗朗西斯菌属（94.8-95.9%）和全弗朗西斯菌属（93.8-94.2%）的成员具有高度同源性。基于16S rRNA基因序列与弗朗西斯菌科成员构建的系统发育树状图显示，SYSU WZ-2菌株形成了一个与弗朗西斯菌属和全弗朗西斯菌属成员明显分开的独特系统发育分支。基质辅助激光解吸电离飞行时间质谱分析也显示，与弗朗西斯菌属和全弗朗西斯菌属成员相比，SYSU WZ-2菌株具有不同的图谱。基于上述结果以及表型和化学分类特征的差异，SYSU WZ-2菌株被鉴定为代表一个新属的新物种，为此提出新属名Pseudofrancisella aestuarii gen. nov., sp. nov.（模式菌株SYSU WZ-2 = KCTC 52557 = CGMCC 1.13718）。
