The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, 510120, People's Republic of China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 Jul;114(7):1079-1089. doi: 10.1007/s10482-021-01579-7. Epub 2021 Apr 24.
Six aerobic Gram-negative bacteria were isolated from seawater in Guangdong Province, P.R. China. Cells were observed to be Gram-negative, aerobic, non-motile and non-spore forming. Growth of the designated type strain 19X3-30 occurred at a temperature range of 14-37 °C (optimum, 28 °C), a pH range of 6.0-8.0 (optimum, pH 7) and up to 7.5% NaCl (optimum, 1.5%; w/v), and was enhanced by CO and L-cysteine supplementation. The major polar lipids identified in strain 19X3-30 were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The principal cellular fatty acids profile showed the presence of anteiso-C, anteiso-C and C (> 8% of total fatty acids), and the respiratory quinone was ubiquinone 8 (UQ-8). According to the analysis of 16S rRNA gene sequences, these strains represented a novel species within the family Fastidiosibacteraceae, sharing maximum similarities with Cysteiniphilum litorale DSM 101832 (96.6%) and Cysteiniphilum halobium DSM 103992 (95.3%). Phylogenetic dendrograms based on 16S rRNA gene and protein marker genes from the genomic sequences both indicated that the strains formed a monophyletic lineage closely linked to the genus Cysteiniphilum, which was also supported by the UPGMA dendrogram based on the MALDI-TOF MS profile. The genomic DNA G + C contents of six strains ranged from 38.0% to 38.1%. Based on different taxonomic genomic metrics, phylogeny and phenotypic features, we propose that the strains warrant the assignment to a novel species, for which the name Cysteiniphilum marinum sp. nov. is proposed. The type strain is 19X3-30 (= KCTC 82154 = CGMCC 1.18585).
从中国广东省海水中共分离到 6 株需氧革兰氏阴性细菌。观察到细胞为革兰氏阴性、需氧、非运动和非孢子形成。指定的模式菌株 19X3-30 的生长温度范围为 14-37°C（最佳温度为 28°C），pH 值范围为 6.0-8.0（最佳 pH 值为 7），最高可达 7.5%NaCl（最佳为 1.5%；w/v），并通过添加 CO 和 L-半胱氨酸增强。在菌株 19X3-30 中鉴定出的主要极性脂质是双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油。主要细胞脂肪酸图谱显示存在 anteiso-C、anteiso-C 和 C（>总脂肪酸的 8%），呼吸醌为泛醌 8（UQ-8）。根据 16S rRNA 基因序列分析，这些菌株代表了 Fastidiosibacteraceae 科中的一个新种，与 Cysteiniphilum litorale DSM 101832（96.6%）和 Cysteiniphilum halobium DSM 103992（95.3%）的相似度最高。基于 16S rRNA 基因和基因组序列中蛋白质标记基因的系统发育树表明，这些菌株形成了一个单系谱系，与 Cysteiniphilum 属密切相关，这也得到了基于 MALDI-TOF MS 图谱的 UPGMA 树的支持。六株菌的基因组 DNA G+C 含量范围为 38.0%-38.1%。基于不同的分类基因组指标、系统发育和表型特征，我们建议这些菌株值得分配到一个新种，提议将其命名为海生硫杆菌属 marinum sp. nov.。模式菌株为 19X3-30（=KCTC 82154=CGMCC 1.18585）。
