Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.
Macromol Biosci. 2017 Sep;17(9). doi: 10.1002/mabi.201700147. Epub 2017 Jul 3.
Silk fibroin (SF) with good biocompatibility and degradability has great potential for tissue engineering. However, the SF based scaffolds lack the electroactivity to regulate the myogenic differentiation for the regeneration of muscle tissue, which is sensitive to electrical signal. Herein, a series of electroactive biodegradable scaffolds based on SF and water-soluble conductive poly(aniline-co-N-(4-sulfophenyl) aniline) (PASA) via a green method for skeletal muscle tissue engineering are designed. SF/PASA scaffolds are prepared by vortex of aqueous solution of SF and PASA under physiological condition. Murine-derived L929 fibroblast and C2C12 myoblast cells are used to evaluate cytotoxicity of SF/PASA scaffolds. Moreover, myogenic differentiation of C2C12 cells is investigated by analyzing the morphology of myotubes and related gene expression. These results suggest that electroactive SF/PASA scaffolds with a suitable microenvironment, which can enhance the myogenic differentiation of C2C12 cells, have a great potential for skeletal muscle regeneration.
丝素蛋白（SF）具有良好的生物相容性和可降解性，在组织工程中有很大的应用潜力。然而，基于 SF 的支架缺乏电活性，无法调节肌肉组织再生的成肌分化，而肌肉组织对电信号很敏感。本文通过一种绿色方法，设计了一系列基于丝素蛋白和水溶性导电聚合物聚（苯胺-co-N-（4-磺苯基）苯胺）（PASA）的电活性可生物降解支架，用于骨骼肌组织工程。SF/PASA 支架是通过在生理条件下将 SF 和 PASA 的水溶液涡旋制备的。使用鼠源性 L929 成纤维细胞和 C2C12 成肌细胞来评估 SF/PASA 支架的细胞毒性。此外，通过分析肌管的形态和相关基因表达来研究 C2C12 细胞的成肌分化。这些结果表明，具有合适微环境的电活性 SF/PASA 支架可以增强 C2C12 细胞的成肌分化，在骨骼肌再生方面具有很大的潜力。
