设计一种用于选择性识别 Au(III)和 Au(I)的双光子荧光探针及其在体外和体内成像中的应用。
Design of a two-photon fluorescent probe for selective recognition of Au(III) over Au(I) and its application of imaging in vitro and in vivo.
机构信息
School of Chemistry and Chemical Engineering & Center for Atomic Engineering of Advanced Materials, Anhui University & AnHui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Hefei 230601, China.
School of Chemistry and Chemical Engineering & Center for Atomic Engineering of Advanced Materials, Anhui University & AnHui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Hefei 230601, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Dec 5;187:110-118. doi: 10.1016/j.saa.2017.06.026. Epub 2017 Jun 20.
A highly selective two-photon fluorescent probe (PyCM-1) for Au was developed with distinct "turn on" fluorescence response, low detection limit (22nM) and large two-photon absorption cross-sections (696 GM at 860nm). Its high selectivity for Au over Au was achieved via the modification on the type of coordination atoms in the Schiff base receptor. Co-staining experiments showed that the probe PyCM-1 could co-localize specifically with mitochondria. Moreover, the two-photon confocal fluorescence imaging results demonstrated the probe's capability for visualizing Auin vitro and in vivo.
一种高选择性的双光子荧光探针（PyCM-1）被开发出来，对 Au 具有明显的“开启”荧光响应，低检测限（22nM）和大的双光子吸收截面（696 GM 在 860nm）。通过修饰席夫碱受体中配位原子的类型，实现了其对 Au 相对于 Au 的高选择性。共染色实验表明，探针 PyCM-1 可以特异性地与线粒体共定位。此外，双光子共聚焦荧光成像结果表明，该探针具有在体外和体内可视化 Au 的能力。
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