Zhang Ya-Jie, Wu Meng-Jun, Yu Hai, Liu Jin
Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
Exp Ther Med. 2017 Jul;14(1):65-72. doi: 10.3892/etm.2017.4446. Epub 2017 May 11.
Emulsified isoflurane (EIso) has a protective effect against ischemia/reperfusion (I/R) injury in animal models. However, the protective effects of EIso on global cerebral I/R injury remain unclear. The present study aimed to investigate whether EIso postconditioning was able to improve survival and neurological outcomes in a rat model of cardiac arrest (CA). Rats were randomly divided into five groups, namely the control, EIso-2ml, EIso-4ml, isoflurane (Iso) and emulsion (E) groups. All rats were resuscitated by a standardized method following 6 min of asphyxia. Furthermore, all interventions were administered immediately following the return of spontaneous circulation (ROSC). The animal survival was recorded daily, and evaluations of behavioral and brain morphology were assessed at 1 and 7 days after ROSC. The results showed that EIso treatment increased the survival rate 7 days after ROSC, with a 41.7% 7-day survival in the EIso-2ml group, 66.7% in the EIso-4ml group and 50% in the Iso group compared with 33.3% survival in the control and E groups. Moreover, the neural deficit score and memory function were improved in the EIso-4ml group, and this treatment also ameliorated brain hippocampal cell injury and apoptosis. In addition, a better brain protective effect was observed in the EIso-4ml group compared with the EIso-2ml, Iso and E groups. In summary, the data of the present study suggest that EIso postconditioning improved the survival and neurological outcomes following CA in a dose-dependent manner.
乳化异氟烷（EIso）在动物模型中对缺血/再灌注（I/R）损伤具有保护作用。然而，EIso对全脑I/R损伤的保护作用仍不清楚。本研究旨在探讨EIso后处理是否能够改善心脏骤停（CA）大鼠模型的存活率和神经功能结局。大鼠被随机分为五组，即对照组、EIso-2ml组、EIso-4ml组、异氟烷（Iso）组和乳剂（E）组。所有大鼠在窒息6分钟后通过标准化方法进行复苏。此外，所有干预措施均在自主循环恢复（ROSC）后立即给予。每天记录动物存活率，并在ROSC后1天和7天评估行为和脑形态。结果显示，EIso处理提高了ROSC后7天的存活率，EIso-2ml组7天存活率为41.7%，EIso-4ml组为66.7%，Iso组为50%，而对照组和E组存活率为33.3%。此外，EIso-4ml组神经功能缺损评分和记忆功能得到改善，该处理还减轻了脑海马细胞损伤和凋亡。此外，与EIso-2ml组、Iso组和E组相比，EIso-4ml组观察到更好的脑保护作用。总之，本研究数据表明，EIso后处理以剂量依赖方式改善了CA后的存活率和神经功能结局。
