乳化异氟醚后处理对大鼠心肌缺血再灌注损伤产生心脏保护作用。
Emulsified isoflurane postconditioning produces cardioprotection against myocardial ischemia-reperfusion injury in rats.
机构信息
Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
出版信息
J Physiol Sci. 2013 Jul;63(4):251-61. doi: 10.1007/s12576-013-0261-z. Epub 2013 Apr 27.
Abstract
Emulsified isoflurane (EIso) preconditioning can induce cardioprotection. We investigated whether EIso application after ischemia protects hearts against reperfusion injury and whether it is mediated by the inhibition of apoptosis. Rats were subjected to 30-min coronary occlusion followed by 180-min reperfusion. At the onset of reperfusion, rats were intravenously administered saline (sham, control group), 30 % intralipid (IL group) or 2 ml kg(-1) EIso (EIso group) for 30 min. After reperfusion, infarct sizes, myocardial apoptosis and expression of Bcl-2, Bax and caspase-3 proteins were determined. Hemodynamic parameters were not different among groups. Compared with control and intralipid group, EIso limited infarct size, inhibited apoptosis, increased the expression of Bcl-2, decreased the expression of Bax, cleaved caspase-3, and enhanced Bcl-2/Bax ratio. EIso protects hearts against reperfusion injury when administered at the onset of reperfusion, which may be mediated by the inhibition of apoptosis via modulation of the expression of pro- and anti-apoptotic proteins.
摘要
乳化异氟醚（EIso）预处理可诱导心脏保护。我们研究了缺血后应用 EIso 是否能保护心脏免受再灌注损伤，以及它是否通过抑制细胞凋亡来介导。大鼠进行 30 分钟的冠状动脉闭塞，然后进行 180 分钟的再灌注。在再灌注开始时，大鼠静脉注射生理盐水（假手术，对照组）、30%脂肪乳剂（IL 组）或 2mlkg(-1)EIso（EIso 组）30 分钟。再灌注后，测定梗死面积、心肌凋亡以及 Bcl-2、Bax 和 caspase-3 蛋白的表达。各组血流动力学参数无差异。与对照组和脂肪乳剂组相比，EIso 可限制梗死面积、抑制凋亡、增加 Bcl-2 表达、降低 Bax 表达、切割 caspase-3，并增强 Bcl-2/Bax 比值。EIso 在再灌注开始时给药可保护心脏免受再灌注损伤，这可能是通过调节促凋亡和抗凋亡蛋白的表达来抑制细胞凋亡介导的。
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