Zhao Jun, Shi Lei, Zhang Li-Rong
Department of Pharmacy, The Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Department of Clinical Pharmacology, The Medical College of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Exp Ther Med. 2017 Jul;14(1):664-670. doi: 10.3892/etm.2017.4571. Epub 2017 Jun 8.
Carnosine is a dipeptide of β-alanine and histidine amino acids. It is widely present in muscle and brain tissues. Carnosine has been demonstrated to be an antioxidant agent that is beneficial in animals. Reactive oxygen species (ROS) and aldehydes are are generated from membrane fatty acid oxidation. The antioxidant potential and toxicity of salsolinol had been extensively studied and . The present study analyzed the protective effect of carnosine against Parkinson's disease in the salsolinol-induced rat brain and rat brain endothelial cells. Antioxidant and biochemical markers were determined and . Histopathological examination was completed in order to evaluate the protective effect of carnosine on the cellular architecture of salsolinol-induced brain tissue. In order to confirm the protective effect of carnosine further, it was also investigated at an level using rat brain endothelial cells. Fluorescence and confocal studies indicated reduced apoptosis in the endothelial cells of the rat brain tissue. Antioxidant enzymes and lipid peroxidation levels were renormalized following treatment with carnosine. In addition, carnosine treatment reduced mitochondria-derived ROS in the rat brain endothelial cells. These findings suggest that carnosine may be a therapeutic agent against salsolinol-induced Parkinson's.
肌肽是β-丙氨酸和组氨酸形成的二肽。它广泛存在于肌肉和脑组织中。已证明肌肽是一种对动物有益的抗氧化剂。活性氧（ROS）和醛类是由膜脂肪酸氧化产生的。对salsolinol的抗氧化潜力和毒性已进行了广泛研究。本研究分析了肌肽对salsolinol诱导的大鼠脑和大鼠脑内皮细胞帕金森病的保护作用。测定了抗氧化和生化标志物。完成了组织病理学检查，以评估肌肽对salsolinol诱导的脑组织细胞结构的保护作用。为了进一步证实肌肽的保护作用，还在大鼠脑内皮细胞水平上进行了研究。荧光和共聚焦研究表明大鼠脑组织内皮细胞凋亡减少。用肌肽处理后，抗氧化酶和脂质过氧化水平恢复正常。此外，肌肽处理降低了大鼠脑内皮细胞中线粒体衍生的ROS。这些发现表明肌肽可能是一种治疗salsolinol诱导的帕金森病的药物。
