Tovar Bohórquez M Oswaldo, Mechaly Alejandro S, Elisio Mariano, Chalde Tomás, Canosa Luis F, Miranda Leandro A, Somoza Gustavo M
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús "Dr. Raúl Alfonsín" (IIB-INTECH), CONICET-UNSAM, Chascomús, Buenos Aires, Argentina.
Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús "Dr. Raúl Alfonsín" (IIB-INTECH), CONICET-UNSAM, Chascomús, Buenos Aires, Argentina.
Gen Comp Endocrinol. 2017 Oct 1;252:209-218. doi: 10.1016/j.ygcen.2017.06.028. Epub 2017 Jun 30.
In vertebrates, the reproduction is controlled by the brain-pituitary-gonadal (BPG) axis and kisspeptin has emerged as a key player of this axis. In this study, we analyzed changes in the expression levels of kiss1, kiss2, and their receptors, kissr2 and kissr3 during gametogenesis in the BPG axis of feral Odontesthes bonariensis. In females, levels of brain kiss1 showed an increase at final maturation (Fm), while kiss2 levels were shown to be high at primary growth (Pg) stage, with no differences in the expression of their receptors. In the pituitary, kiss1 and kiss2 peaked at the cortical alveoli (Ca) stage, and kissr3 at initial vitellogenesis. In parallel, there was an increase of kiss1, kissr2 and kissr3 in the ovary during the Ca stage and both receptors again at Fm stage. In males, the four genes were highly expressed in the brain at the arrested (A) stage. In the pituitary, kiss2 peaked at spermatogonial (SG) and spermatocytary (SC) stages; while kissr3 reached a peak at the spermiogenic stage (SP). In testes, kiss1 and kiss2 significantly increased during the SG and SC stages; meanwhile, kissr2 increased at SG and SC, whereas kissr3 levels were significantly high at SC and SP stages. Taken together these results showed that the kisspeptin system in pejerrey is expressed in the three levels of the BPG axis with different expression profiles during the gonadal cycle. These findings pointed that kisspeptins have different roles in gametogenesis in this species.
在脊椎动物中，生殖受脑-垂体-性腺（BPG）轴调控，而 kisspeptin 已成为该轴的关键调节因子。在本研究中，我们分析了野生博氏半线脂鲤 BPG 轴配子发生过程中 kiss1、kiss2 及其受体 kissr2 和 kissr3 的表达水平变化。在雌性中，脑 kiss1 水平在最终成熟（Fm）时升高，而 kiss2 水平在初级生长（Pg）阶段较高，其受体表达无差异。在垂体中，kiss1 和 kiss2 在皮质泡（Ca）阶段达到峰值，kissr3 在卵黄发生初期达到峰值。同时，卵巢中 kiss1、kissr2 和 kissr3 在 Ca 阶段增加，两种受体在 Fm 阶段再次增加。在雄性中，这四个基因在静止（A）阶段的脑中高表达。在垂体中，kiss2 在精原细胞（SG）和精母细胞（SC）阶段达到峰值；而 kissr3 在精子发生阶段（SP）达到峰值。在睾丸中，kiss1 和 kiss2 在 SG 和 SC 阶段显著增加；同时，kissr2 在 SG 和 SC 阶段增加，而 kissr3 水平在 SC 和 SP 阶段显著升高。综合这些结果表明，佩氏南美脂鲤的 kisspeptin 系统在 BPG 轴的三个水平上表达，在性腺周期中具有不同的表达模式。这些发现表明 kisspeptins 在该物种的配子发生中具有不同作用。
