产油真菌高山被孢霉的代谢工程改造以提高油酸和亚油酸的产量。
Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids.
机构信息
Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8513, Japan.
Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8513, Japan.
出版信息
Bioresour Technol. 2017 Dec;245(Pt B):1610-1615. doi: 10.1016/j.biortech.2017.06.089. Epub 2017 Jun 19.
The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain.
本工作旨在研究产油丝状霉菌白黄丝霉菌的分子育种，以提高亚油酸（LA）或油酸（OA）的产量。来源于毛头鬼伞的 Δ12-去饱和酶（DS）基因在白黄丝霉菌 Δ6DS 活性缺陷突变体中的异源表达，使 LA 产量相对于总脂肪酸增加到野生型的 5 倍。通过在白黄丝霉菌 Δ12DS 活性缺陷突变体中通过 RNAi 抑制内源性 Δ6I 基因的表达，OA 相对于总脂肪酸的积累率达到 68.0%。这些转化体的 LA 和 OA 产量在第 5 天分别达到 1.44 和 2.76g/L。RNAi 处理菌株的 Δ6I 转录水平抑制到亲本菌株的 1/10。Δ6I RNAi 处理菌株中的 Δ6II RNA 量增加到野生型的 8 倍。
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