Utispan Kusumawadee, Chitkul Bordin, Monthanapisut Paopanga, Meesuk Ladda, Pugdee Kamolparn, Koontongkaew Sittichai
Oral Health Prev Dent. 2017;15(3):279-284. doi: 10.3290/j.ohpd.a38528.
The aim of this study was to determine the antimicrobial effects of propolis extracted from an endemic species of stingless bee, T. sirindhornae, on the cariogenic bacterium Streptococcus mutans.
Dichloromethane extracts (DME) of propolis (DMEP) were prepared and analysed by reverse-phase high-performance liquid chromatography. The antibacterial growth and antibiofilm formation effects of DMEP on S. mutans were compared with those of apigenin, a commercial propolis product. The effects of DMEP and apigenin on glucosyltransferase (gtf) B expression in S. mutans were investigated using real-time polymerase chain reaction. Chlorhexidine (CHX) was used as a positive control in the experiments.
Apigenin, pinocembrin, p-coumaric acid, and caffeic acid were not detected in the propolis extracts. DMEP and apigenin significantly inhibited S. mutans growth (IC50 = 43.5 and 17.36 mg/ml, respectively). DMEP and apigenin also exhibited antiadherence effects on S. mutans as shown by reduced biofilm formation. Furthermore, a significant inhibition in gtfB expression was observed in DMEP and apigenin treated S. mutans.
Propolis produced by T. sirindhornae demonstrated antibacterial and antibiofilm effects, and reduced gtfB expression in S. mutans. The antibacterial activities of propolis observed were not due to apigenin, pinocembrin, p-coumaric acid, or caffeic acid.
本研究旨在确定从无刺蜂本地物种西里伯斯蜜环蜂（T. sirindhornae）中提取的蜂胶对致龋菌变形链球菌的抗菌作用。
制备蜂胶的二氯甲烷提取物（DME），并通过反相高效液相色谱法进行分析。将DME对变形链球菌的抗菌生长和抗生物膜形成作用与芹菜素（一种商业蜂胶产品）进行比较。使用实时聚合酶链反应研究DME和芹菜素对变形链球菌中葡糖基转移酶（gtf）B表达的影响。实验中使用氯己定（CHX）作为阳性对照。
在蜂胶提取物中未检测到芹菜素、松属素、对香豆酸和咖啡酸。DME和芹菜素显著抑制变形链球菌生长（IC50分别为43.5和17.36 mg/ml）。DME和芹菜素还对变形链球菌表现出抗黏附作用，生物膜形成减少。此外，在经DME和芹菜素处理的变形链球菌中观察到gtfB表达受到显著抑制。
西里伯斯蜜环蜂产生的蜂胶具有抗菌和抗生物膜作用，并降低了变形链球菌中gtfB的表达。观察到的蜂胶抗菌活性并非由芹菜素、松属素、对香豆酸或咖啡酸引起。
