State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
Antimicrob Agents Chemother. 2020 Aug 20;64(9). doi: 10.1128/AAC.00251-20.
Dental caries is the most common disease in the human mouth. is the primary cariogenic bacterium. Propolis is a nontoxic natural product with a strong inhibitory effect on oral cariogenic bacteria. The polyphenol-rich extract from propolis inhibits growth and biofilm formation, as well as the genes involved in virulence and adherence, through the inhibition of glucosyltransferases (GTF). However, because the chemical composition of propolis is highly variable and complex, the mechanism of its antimicrobial action and the active compound are controversial and not completely understood. Caffeic acid phenethyl ester (CAPE) is abundant in the polyphenolic compounds from propolis, and it has many pharmacological effects. In this study, we investigated the antibacterial effects of CAPE on common oral cariogenic bacteria (, , , and ) and its effects on the biofilm-forming and cariogenic abilities of CAPE shows remarkable antimicrobial activity against cariogenic bacteria. Moreover, CAPE also inhibits the formation of biofilms and their metabolic activity in mature biofilms. Furthermore, CAPE can inhibit the key virulence factors of associated with cariogenicity, including acid production, acid tolerance, and the bacterium's ability to produce extracellular polysaccharides (EPS), without affecting bacterial viability at subinhibitory levels. In conclusion, CAPE appears to be a new agent with anticariogenic potential, not only via inhibition of the growth of cariogenic bacteria.
龋齿是人类口腔中最常见的疾病。 是主要的致龋菌。蜂胶是一种无毒的天然产物，对口腔致龋菌具有很强的抑制作用。蜂胶中富含多酚的提取物通过抑制葡糖基转移酶（GTF）来抑制 生长和生物膜形成，以及与毒力和黏附相关的基因。然而，由于蜂胶的化学成分高度可变且复杂，其抗菌作用机制和有效化合物存在争议，尚未完全阐明。咖啡酸苯乙酯（CAPE）是蜂胶中多酚类化合物的丰富成分，具有许多药理学作用。在这项研究中，我们研究了 CAPE 对常见口腔致龋菌（ 、 、 、 ）的抗菌作用及其对 生物膜形成和致龋能力的影响。CAPE 对致龋菌表现出显著的抗菌活性。此外，CAPE 还能抑制 生物膜的形成及其在成熟生物膜中的代谢活性。此外，CAPE 可以抑制与致龋性相关的 关键毒力因子的产生，包括产酸、耐酸和细菌产生细胞外多糖（EPS）的能力，而在亚抑菌水平下不影响细菌活力。总之，CAPE 似乎是一种具有抗龋潜力的新型药物，不仅通过抑制致龋菌的生长。
