Abayli Hasan, Tonbak Sukru, Azkur Ahmet Kursat, Bulut Hakan
Department of Virology, Faculty of Veterinary Medicine, Firat University, 23110, Elazig, Turkey.
Department of Virology, Faculty of Veterinary Medicine, Kirikkale University, 71450, Kirikkale, Turkey.
Arch Virol. 2017 Oct;162(10):3233-3238. doi: 10.1007/s00705-017-3470-6. Epub 2017 Jul 3.
Relatively high prevalence and mortality rates of bovine ephemeral fever (BEF) have been reported in recent epidemics in some countries, including Turkey, when compared with previous outbreaks. A limited number of complete genome sequences of BEF virus (BEFV) are available in the GenBank Database. In this study, the complete genome of highly pathogenic BEFV isolated during an outbreak in Turkey in 2012 was analyzed for genetic characterization. The complete genome of the Turkish BEFV isolate was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced. It was found that the complete genome of the Turkish BEFV isolate was 14,901 nt in length. The complete genome sequence obtained from the study showed 91-92% identity at nucleotide level to Australian (BB7721) and Chinese (Bovine/China/Henan1/2012) BEFV isolates. Phylogenetic analysis of the glycoprotein gene of the Turkish BEFV isolate also showed that Turkish isolates were closely related to Israeli isolates. Because of the limited number of complete BEFV genome sequences, the results from this study will be useful for understanding the global molecular epidemiology and geodynamics of BEF.
与以往疫情相比，近期在包括土耳其在内的一些国家暴发的牛流行热（BEF）疫情中，报告了相对较高的发病率和死亡率。GenBank数据库中可获得的牛流行热病毒（BEFV）完整基因组序列数量有限。在本研究中，对2012年土耳其一次疫情期间分离出的高致病性BEFV的完整基因组进行了遗传特征分析。通过逆转录-聚合酶链反应（RT-PCR）扩增并测序了土耳其BEFV分离株的完整基因组。结果发现，土耳其BEFV分离株的完整基因组长度为14901个核苷酸。该研究获得的完整基因组序列在核苷酸水平上与澳大利亚（BB7721）和中国（牛/中国/河南1/2012）BEFV分离株具有91%-92%的同一性。对土耳其BEFV分离株糖蛋白基因的系统发育分析也表明，土耳其分离株与以色列分离株密切相关。由于完整的BEFV基因组序列数量有限，本研究结果将有助于了解BEF的全球分子流行病学和地理动态。
