Kun Jia, Rongrong Jia, Xiangbin Wang, Yan Zhao, Yiping Dou, Gang Lu, Pei Zhou, Shoujun Li
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, People's Republic of China.
Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, 510642, People's Republic of China.
Virus Genes. 2020 Jun;56(3):390-395. doi: 10.1007/s11262-020-01740-w. Epub 2020 Feb 6.
Bovine ephemeral fever virus (BEFV) can cause bovine ephemeral fever and is an economically important arbovirus of cattle. To expand the knowledge of the molecular epidemiology of BEFV in southern China, the complete surface glycoprotein G gene of BEFV was sequenced from samples collected in five restricted outbreaks from 2013 to 2017, namely 2013ZH, 2014HM, 2015GX, 11082-2016, and qy2017. It was noted that both 2014HM and 11082-2016 were detected in cattle regularly vaccinated with inactivated vaccine. Phylogenetic analysis demonstrated that all five strains grouped into cluster I. However, qy2017 was closer to the BEFV strains identified in Thailand, Japan, and Taiwan after 2000, while 2013ZH, 2014HM, 2015GX, and 11082-2016 were closer to the Chinese strains in 2011 and the Turkey strains in 2012. The analysis of antigenic sites indicated that several amino acid changes occurred between the five strains and the vaccine strain. Importantly, one novel amino acid mutation site was observed in the putative N-linked glycosylation sites of 2013ZH, 2014HM, 2015GX, and 11082-2016. Our study indicated novel genetic characteristics of the newly emerging BEFV strains in southern China and the necessity of updating the component of commercially available inactivated BEFV vaccines in China.
牛暂时热病毒（BEFV）可引发牛暂时热，是一种对养牛业具有重要经济影响的虫媒病毒。为拓展对中国南方BEFV分子流行病学的认识，对2013年至2017年期间从5次局部疫情中采集的样本进行了BEFV表面糖蛋白G基因全序列测序，这5次疫情分别为201️3ZH、2014HM、2015GX、11082 - 2016和qy2017。值得注意的是，2014HM和11082 - 2016是在定期接种灭活疫苗的牛群中检测到的。系统发育分析表明，所有5个毒株均归为第一簇。然而，qy2017与2000年后在泰国、日本和台湾地区鉴定出的BEFV毒株更为接近，而2013ZH、2014HM、2015GX和11082 - 2016则与2011年的中国毒株以及2012年的土耳其毒株更为接近。抗原位点分析表明，这5个毒株与疫苗毒株之间发生了若干氨基酸变化。重要的是，在2013ZH、2014HM、2015GX和11082 - 2016的推定N - 糖基化位点中观察到一个新的氨基酸突变位点。我们的研究揭示了中国南方新出现的BEFV毒株的新遗传特征，以及更新中国市售BEFV灭活疫苗成分的必要性。
