Department of Animal Virology, Research and Diagnosis, Agricultural Research, Education and Organization (AREEO), Razi Vaccine and Serum Research Institute, P.O. Box: 31975/148, Karaj, Iran.
Veterinary Clinic, Shariati Street, Tehran, Iran.
Arch Microbiol. 2023 Apr 16;205(5):196. doi: 10.1007/s00203-023-03527-7.
Bovine ephemeral fever virus (BEFV) is an economically important arthropod-borne virus of cattle and water buffaloes which is enzootic in Africa, Australia, and Asia. We characterized the entire length of BEFV BA/RZ/IR strain genome isolated in Iran and compared to the all BEFV full genomes available in the GenBank. The BEFV genomes were phylogenetically classified as 4 lineages including the Middle Eastern, East Asian, Australian, and South African lineages. The Iranian BA/RZ/IR strain, which displayed maximum sequence identity (96.72%) to the Chinese JT02L strain was clustered as a separate branch in the East Asian lineage of the virus. Using Shannon entropy analysis, amino acid variations were detected in the all proteins encoded by BEFV genomes. Particularly, the polymerase L and the accessory proteins Gns, α2 and β exhibited the highest amino acid variations suggesting their significance in the viral replication efficiency. Our bioinformatics analyses also predict the occurrence of recombination event within the East Asian lineage of BEFV genomes. Our data show that the Chinese Henan 1 may be a hybrid strain constructed of the Chinese JT02L and Iranian BA/RZ/IR BEFV strains as the major and minor parents, respectively. These computational analyses suggest that the homologous recombination may be an evolutionary mechanism for BEFV as a member of the Rhabdoviridae family.
牛暂时热病毒（BEFV）是一种经济上重要的节肢动物传播的病毒，感染牛和水牛，在非洲、澳大利亚和亚洲流行。我们对在伊朗分离的 BEFV BA/RZ/IR 株的全长基因组进行了特征描述，并与 GenBank 中所有 BEFV 全基因组进行了比较。BEFV 基因组在系统发育上分为 4 个谱系，包括中东、东亚、澳大利亚和南非谱系。伊朗的 BA/RZ/IR 株与中国的 JT02L 株具有最大的序列同一性（96.72%），被聚类为病毒东亚谱系中的一个单独分支。使用香农熵分析，在 BEFV 基因组编码的所有蛋白质中检测到氨基酸变异。特别是聚合酶 L 和辅助蛋白 Gns、α2 和 β 表现出最高的氨基酸变异，表明它们在病毒复制效率中的重要性。我们的生物信息学分析还预测了 BEFV 东亚谱系基因组内发生重组事件的可能性。我们的数据表明，中国河南 1 号可能是由中国 JT02L 和伊朗 BA/RZ/IR BEFV 株作为主要和次要亲本构建的杂种株。这些计算分析表明，同源重组可能是 Rhabdoviridae 家族成员 BEFV 的一种进化机制。
