Gu Hongbo, Ren Jianmin, Jia Xiaoying, Stokes Matthew P
Proteomic Service Group, Cell Signaling Technology, 3 Trask Lane, Danvers, MA, 01923, USA.
Methods Mol Biol. 2017;1619:119-125. doi: 10.1007/978-1-4939-7057-5_9.
Immunoaffinity enrichment combined with LC-MS/MS enables identification of Post-translational modifications (PTMs) from serum/plasma samples without abundant protein depletion. Here we described the workflow in details in identifying various types of PTMs such as lysine acetylation and arginine methylation from cancer serum. The method described is compatible with all common proteomic analysis platforms and quantitative methods.
免疫亲和富集结合液相色谱-串联质谱法能够在不进行大量蛋白质去除的情况下，从血清/血浆样本中鉴定翻译后修饰（PTM）。在此，我们详细描述了从癌症血清中鉴定各种类型PTM（如赖氨酸乙酰化和精氨酸甲基化）的工作流程。所描述的方法与所有常见的蛋白质组学分析平台和定量方法兼容。
