Kisrieva Y S, Petushkova N A, Samenkova N F, Kuznetsova G P, Larina O B, Teryaeva N B, Usachev D Yu, Zgoda V G, Karuzina I I
Institute of Biomedical Chemistry, Moscow, Russia.
Burdenko Institute of Neurosurgery, Moscow, Russia.
Biomed Khim. 2019 Apr;65(3):251-258. doi: 10.18097/PBMC20196503251.
The relative differences between post-translational modifications (PTM) of proteins in blood plasma samples of patients with cerebral ischemia (CI) and healthy people were investigated using of the method of label-free comparative proteomic analysis based on the technology of tandem HPLC-MS/MS. For PTM detection we used multiple MS/MS search in the database Mascot for variable PTM and Progenesis LS-MS software. In the CI plasma samples, we observed an increase in the proportion of peptides with such PTM as phosphorylation of serine, threonine, and tyrosine, acetylation of lysine and protein N-term, ubiquitination of lysine and deamidation of glutamine related to clinically significant processes were revealed.
采用基于串联HPLC-MS/MS技术的无标记比较蛋白质组学分析方法，研究了脑缺血（CI）患者与健康人血浆样本中蛋白质翻译后修饰（PTM）的相对差异。对于PTM检测，我们在数据库Mascot中使用了针对可变PTM的多次MS/MS搜索以及Progenesis LS-MS软件。在CI血浆样本中，我们观察到与临床显著过程相关的丝氨酸、苏氨酸和酪氨酸磷酸化、赖氨酸和蛋白质N端乙酰化、赖氨酸泛素化以及谷氨酰胺脱酰胺等PTM肽段比例增加。
