a Department of Neurosurgery , Qilu Hospital of Shandong University , Jinan , Shandong , China.
b Department of Neurosurgery , Yantaishan Hospital , Yantai , Shandong , China.
Artif Cells Nanomed Biotechnol. 2018 Jun;46(4):815-818. doi: 10.1080/21691401.2017.1345923. Epub 2017 Jul 4.
Recently, microRNA-133b (miR-133b) dysregulation has been shown to play a key role in several human cancers, as well as glioma. In this study, we aimed to investigate the clinical significance and prognostic value of miR-133b in glioma.
Real-time quantitative PCR was employed to measure the expression level of miR-133b in tissues. Survival analysis was carried out by using the log-rank test and Kaplan-Meier method. Prognostic factors for overall survival were identified by univariate and multivariate analyses using the Cox proportional hazards regression model.
The expression level of miR-133b was significantly lower in glioma tissues compared with matched non-cancerous brain tissues (p < .05). Its level was strongly correlated with Karnofsky Performance Scale score (p < .001) and WHO grade (p < .001). Kaplan-Meier survival and log-rank analysis indicated that the decreased expression of miR-133b was strongly correlated with shorter overall survival of patients with glioma (log-rank test, p = .03).
The current investigation demonstrated that miR-133b level is useful for predicting the prognosis of patients with glioma.
最近的研究表明，微小 RNA-133b（miR-133b）失调在多种人类癌症以及神经胶质瘤中起着关键作用。在这项研究中，我们旨在探讨 miR-133b 在神经胶质瘤中的临床意义和预后价值。
采用实时定量 PCR 检测组织中 miR-133b 的表达水平。采用对数秩检验和 Kaplan-Meier 方法进行生存分析。采用 Cox 比例风险回归模型进行单因素和多因素分析，确定总生存期的预后因素。
与配对的非癌性脑组织相比，神经胶质瘤组织中 miR-133b 的表达水平显著降低（p<.05）。其水平与卡氏功能状态评分（Karnofsky Performance Scale，KPS）（p<.001）和世界卫生组织（World Health Organization，WHO）分级（p<.001）强烈相关。Kaplan-Meier 生存和对数秩分析表明，miR-133b 表达降低与神经胶质瘤患者的总生存期缩短密切相关（对数秩检验，p=.03）。
本研究表明 miR-133b 水平可用于预测神经胶质瘤患者的预后。
