基于免疫受体酪氨酸的抑制性基序（ITIM）的酶促自组装。
Enzymatic self-assembly of an immunoreceptor tyrosine-based inhibitory motif (ITIM).
作者信息
Yamagata Natsuko, Chen Xiaoyi, Zhou Jie, Li Jie, Du Xuewen, Xu Bing
机构信息
Department of Chemistry, Brandeis University, 415 South Street, Waltham, Massachusetts 02454, USA.
出版信息
Org Biomol Chem. 2017 Jul 21;15(27):5689-5692. doi: 10.1039/c7ob01074e. Epub 2017 Jul 4.
Abstract
Here we show the first example of an immunoreceptor tyrosine-based inhibitory motif (ITIM), LYYYYL, as well as its enantiomeric or retro-inverso peptide, to self-assemble in water via enzyme-instructed self-assembly. Upon enzymatic dephosphorylation, the phosphohexapeptides become hexapeptides, which self-assemble in water to result in supramolecular hydrogels. This work illustrates a new approach to design bioinspired soft materials from a less explored, but important pool of immunomodulatory peptides.
摘要
在此，我们展示了首个基于免疫受体酪氨酸的抑制性基序（ITIM）——LYYYYL，及其对映体或逆序肽通过酶促自组装在水中自组装的实例。经酶促去磷酸化后，磷酸化六肽变成六肽，其在水中自组装形成超分子水凝胶。这项工作阐明了一种从研究较少但重要的免疫调节肽库中设计受生物启发的软材料的新方法。
相似文献
1
Enzymatic self-assembly of an immunoreceptor tyrosine-based inhibitory motif (ITIM).
Org Biomol Chem. 2017 Jul 21;15(27):5689-5692. doi: 10.1039/c7ob01074e. Epub 2017 Jul 4.
2
Enzyme-Instructed Self-Assembly of Small D-Peptides as a Multiple-Step Process for Selectively Killing Cancer Cells.
J Am Chem Soc. 2016 Mar 23;138(11):3813-23. doi: 10.1021/jacs.5b13541. Epub 2016 Mar 11.
3
Microglial activatory (immunoreceptor tyrosine-based activation motif)- and inhibitory (immunoreceptor tyrosine-based inhibition motif)-signaling receptors for recognition of the neuronal glycocalyx.
Glia. 2013 Jan;61(1):37-46. doi: 10.1002/glia.22359. Epub 2012 May 21.
4
Fcgamma receptor-mediated inhibition of human B cell activation: the role of SHP-2 phosphatase.
Eur J Immunol. 1999 Jun;29(6):1980-9. doi: 10.1002/(SICI)1521-4141(199906)29:06<1980::AID-IMMU1980>3.0.CO;2-B.
5
Branched peptides for enzymatic supramolecular hydrogelation.
Chem Commun (Camb). 2017 Dec 19;54(1):86-89. doi: 10.1039/c7cc08421h.
6
Enzyme-Instructed Self-Assembly Enabled Monomer-Excimer Transition to Construct Higher Ordered Luminescent Supramolecular Assembly for Activity-based Bioimaging.
Angew Chem Int Ed Engl. 2021 Apr 6;60(15):8121-8129. doi: 10.1002/anie.202014278. Epub 2021 Mar 3.
7
D-amino acids modulate the cellular response of enzymatic-instructed supramolecular nanofibers of small peptides.
Biomacromolecules. 2014 Oct 13;15(10):3559-68. doi: 10.1021/bm5010355. Epub 2014 Sep 17.
8
Testing the role of the FcγRIIB immunoreceptor tyrosine-based inhibitory motif in regulation of the B cell immune response.
Immun Inflamm Dis. 2015 Sep;3(3):247-64. doi: 10.1002/iid3.64. Epub 2015 Jun 4.
9
Integrating Enzymatic Self-Assembly and Mitochondria Targeting for Selectively Killing Cancer Cells without Acquired Drug Resistance.
J Am Chem Soc. 2016 Dec 14;138(49):16046-16055. doi: 10.1021/jacs.6b09783. Epub 2016 Dec 1.
10
Immunoreceptor tyrosine-based inhibitory motif-dependent functions of an MHC class I-specific NK cell receptor.
Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):E8440-E8447. doi: 10.1073/pnas.1713064114. Epub 2017 Sep 18.
引用本文的文献
1
Enzymatic Noncovalent Synthesis.
Chem Rev. 2020 Sep 23;120(18):9994-10078. doi: 10.1021/acs.chemrev.0c00306. Epub 2020 Aug 19.
2
Enzymatic Noncovalent Synthesis of Supramolecular Soft Matter for Biomedical Applications.
Matter. 2019 Nov 6;1(5):1127-1147. doi: 10.1016/j.matt.2019.09.015.
本文引用的文献
1
Heterotypic Supramolecular Hydrogels.
J Mater Chem B. 2016 Sep 14;4(34):5638-5649. doi: 10.1039/C6TB01592A. Epub 2016 Jul 26.
2
In vitro drug release and percutaneous behavior of poloxamer-based hydrogel formulation containing traditional Chinese medicine.
Colloids Surf B Biointerfaces. 2016 Dec 1;148:526-532. doi: 10.1016/j.colsurfb.2016.09.036. Epub 2016 Sep 23.
3
Microfabrication of a tunable collagen/alginate-chitosan hydrogel membrane for controlling cell-cell interactions.
Carbohydr Polym. 2016 Nov 20;153:652-662. doi: 10.1016/j.carbpol.2016.07.058. Epub 2016 Jul 18.
4
Engineering Immunomodulatory Biomaterials To Tune the Inflammatory Response.
Trends Biotechnol. 2016 Jun;34(6):470-482. doi: 10.1016/j.tibtech.2016.03.009. Epub 2016 Apr 29.
5
Minimal C-terminal modification boosts peptide self-assembling ability for necroptosis of cancer cells.
Chem Commun (Camb). 2016 May 7;52(37):6332-5. doi: 10.1039/c6cc02282k. Epub 2016 Apr 18.
6
Fabrication of a Micellar Supramolecular Hydrogel for Ocular Drug Delivery.
Biomacromolecules. 2016 Mar 14;17(3):798-807. doi: 10.1021/acs.biomac.5b01526. Epub 2016 Feb 12.
7
Virus-like nanostructures for tuning immune response.
Sci Rep. 2015 Nov 18;5:16728. doi: 10.1038/srep16728.
8
Chitosan thermogels for local expansion and delivery of tumor-specific T lymphocytes towards enhanced cancer immunotherapies.
Biomaterials. 2016 Jan;75:237-249. doi: 10.1016/j.biomaterials.2015.10.021. Epub 2015 Oct 9.
9
Enzyme-instructed self-assembly: a multistep process for potential cancer therapy.
Bioconjug Chem. 2015 Jun 17;26(6):987-99. doi: 10.1021/acs.bioconjchem.5b00196. Epub 2015 May 14.
10
Vaccine self-assembling immune matrix is a new delivery platform that enhances immune responses to recombinant HBsAg in mice.
Clin Vaccine Immunol. 2015 Mar;22(3):336-43. doi: 10.1128/CVI.00714-14. Epub 2015 Jan 21.
Zotero 插件