a Department of Drug Technology and Social Pharmacy , Lithuanian University of Health Sciences, Medical academy , Kaunas , Lithuania.
b Department of Analytical and Toxicological Chemistry , Lithuanian University of Health Sciences, Medical Academy , Kaunas , Lithuania.
Pharm Dev Technol. 2018 Jul;23(6):636-645. doi: 10.1080/10837450.2017.1350981. Epub 2017 Jul 14.
For the last couple of decades, multiple emulsions were prepared either by the re-emulsification of primary emulsion or they were produced by an emulsion inversion and their technological peculiarities were widely investigated. The aim of our study was to investigate and determine the optimal technological parameters of innovative multiple emulsion, prepared directly-by addition of ethanolic rosemary extract in the presence of polymeric emulsifier-and evaluate its stability by experimental surface response design approach. The results revealed that simplified W/O/W emulsification process is stirring time and stirring speed sensitive: the change of stirring time from 5 to 15 min at 600 rpm resulted in increased viscosity (from 1705.6 ± 62.2 to 3364.1 ± 112.5 mPA/s) and smaller oil droplet size (from 33.09 ± 1.51 to 17.81 ± 0.78 μm), though the conductivity increased from 800 ± 2 to 882 ± 2 μS/cm (p < .05). The second mixing stage (1000 rpm) had a negative effect on the conductivity of W/O/W emulsion because of the inner aqueous phase encapsulation efficiency. Ethanolic rosemary extract was used as multifunctional agent: not only to form multiple emulsion but also to preserve it; microbiological assay confirmed its effectiveness. A stable W/O/W type drug delivery system was successfully created without additional technological stages, phase inversion or surfactants.
在过去的几十年中，人们通过重新乳化初级乳液或通过乳液反转来制备多重乳液，并广泛研究了它们的技术特点。我们的研究目的是通过实验表面响应设计方法来研究和确定直接通过添加醇提迷迭香提取物在存在聚合物乳化剂的情况下制备的创新型多重乳液的最佳工艺参数，并评估其稳定性。结果表明，简化的 W/O/W 乳化过程对搅拌时间和搅拌速度敏感：搅拌时间从 5 分钟增加到 15 分钟（600rpm），导致粘度增加（从 1705.6±62.2 增加到 3364.1±112.5mPA/s），油滴尺寸减小（从 33.09±1.51 减小到 17.81±0.78μm），而电导率从 800±2 增加到 882±2μS/cm（p<0.05）。第二混合阶段（1000rpm）由于内水相包封效率对 W/O/W 乳液的电导率产生负面影响。醇提迷迭香提取物用作多功能试剂：不仅可以形成多重乳液，还可以保存它；微生物测定证实了其有效性。无需额外的技术阶段、相反转或表面活性剂，成功地创建了稳定的 W/O/W 型药物传递系统。
