Nemickaite Emilija, Zlabiene Ugne, Mazurkeviciute Agne, Marksa Mindaugas, Bernatoniene Jurga
Department of Drug Technology and Social Pharmacy, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania.
Institute of Pharmaceutical Technologies, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania.
Pharmaceutics. 2025 Feb 28;17(3):309. doi: 10.3390/pharmaceutics17030309.
Chitosan-alginate microcapsules were produced to encapsulate bioactive compounds from L. extract (apigenin, luteolin) and cannabidiol (CBD). The study aimed to optimize emulsion composition and encapsulation parameters for potential applications in food supplements and pharmaceuticals. A water-in-oil-in-water (W/O/W) emulsion and a modified coacervation extrusion technique were employed. The study was conducted in two phases using response surface methodology. Key metrics included encapsulation efficiency (EE), yield (EY), cumulative release in vitro, and physicochemical and morphological properties, analyzed via scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), high-performance liquid chromatography with a diode array detector (HPLC-DAD), and gas chromatography with flame ionization detection (GC-FID). The optimal conditions were identified as 0.1% Tween 20, 3.8% Span 80, 3.8% CBD, 19.9% L. extract, 1.5% outer-phase Tween 20, 48.5% sodium alginate, 200 rpm stirring for 30 min, and a 0.05 mL/min flow rate. The EE values were 80.32 ± 4.11% for CBD, 88.13 ± 3.13% for apigenin, and 88.41 ± 4.17% for luteolin, with respective cumulative releases of 77.18 ± 4.4%, 75.12 ± 4.81%, and 75.32 ± 4.53%. The developed microcapsules demonstrated high encapsulation efficiency and controlled release, highlighting their potential for further development in food supplements and pharmaceuticals. Future studies should focus on refining the formulation for improved bioavailability and stability.
制备了壳聚糖-海藻酸盐微胶囊，用于包封来自L.提取物（芹菜素、木犀草素）和大麻二酚（CBD）的生物活性化合物。该研究旨在优化乳液组成和包封参数，以用于食品补充剂和药物的潜在应用。采用了水包油包水（W/O/W）乳液和改进的凝聚挤出技术。该研究使用响应面法分两个阶段进行。关键指标包括包封效率（EE）、产率（EY）、体外累积释放率，以及通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、带二极管阵列检测器的高效液相色谱（HPLC-DAD）和火焰离子化检测气相色谱（GC-FID）分析的物理化学和形态学性质。确定的最佳条件为0.1%吐温20、3.8%司盘80、3.8% CBD、19.9% L.提取物、1.5%外相吐温20、48.5%海藻酸钠、200 rpm搅拌30分钟和0.05 mL/min的流速。CBD的EE值为80.32±4.11%，芹菜素为88.13±3.13%，木犀草素为88.41±4.17%，各自的累积释放率分别为77.18±4.4%、75.12±4.81%和75.32±4.53%。所制备的微胶囊表现出高包封效率和控释性能，突出了它们在食品补充剂和药物中进一步开发的潜力。未来的研究应集中在优化配方以提高生物利用度和稳定性上。
