CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
CEB-Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Sci Total Environ. 2017 Dec 15;605-606:677-682. doi: 10.1016/j.scitotenv.2017.06.216. Epub 2017 Jul 1.
This work demonstrates that cork used as oil-spill sorbents, contaminated with liquid hydrocarbons, herein demonstrated with hexadecane, can be biologically treated by Rhodococcus opacus B4 with concomitant lipids production. R. opacus B4 consumed up to 96% of hexadecane (C16) impregnated in natural and regranulated cork sorbents after 48h incubation, producing 0.59±0.06g of triacylglycerol (TAG) g of C16 consumed with a TAG content of 0.60±0.06gg of cellular dry weight (CDW) and 0.54±0.05g TAG g of C16 consumed with a TAG content of 0.77±0.04gg (CDW), respectively. TAG was mainly composed by fatty acids of 16 and 18 carbon chains demonstrating the feasibility of using it as raw material for biodiesel production. In addition, the obtained lipid-rich biomass (whole cells) can be used for biomethane production, at a yield of 0.4L CH g (CDW). The obtained results support a novel approach for management of oil-spill contaminated cork sorbents through its valorisation by producing bacterial lipids, which can be used as feedstocks for biofuels production.
这项工作表明，被液体烃类污染的用作溢油吸附剂的软木[1]，用十六烷[2]在此处进行了演示，可以被红球菌属（Rhodococcus opacus）B4 进行生物处理，同时产生脂质。在 48 小时孵育后，R. opacus B4 消耗了天然和再造软木吸附剂中多达 96%的十六烷（C16），消耗每克 C16 可产生 0.59±0.06 克三酰基甘油（TAG），TAG 含量为 0.60±0.06gg 细胞干重（CDW），消耗每克 C16 可产生 0.54±0.05 克 TAG，TAG 含量为 0.77±0.04gg（CDW）。TAG 主要由 16 和 18 个碳链的脂肪酸组成，证明了将其用作生物柴油生产原料的可行性。此外，获得的富含脂质的生物质（全细胞）可用于生物甲烷生产，产甲烷量为 0.4L CH g（CDW）。所得结果支持了一种通过生产细菌脂质来实现溢油污染软木吸附剂增值的新方法，这些脂质可用作生物燃料生产的原料。
[1] cork: 软木
[2] hexadecane: 十六烷
