Wei Zhen, Zeng Guangming, Kosa Matyas, Huang Danlian, Ragauskas Arthur J
College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People's Republic of China.
Appl Biochem Biotechnol. 2015 Jan;175(2):1234-46. doi: 10.1007/s12010-014-1305-4. Epub 2014 Nov 7.
Light oil from pyrolysis, which accounts for ∼10 % carbon yield of the starting biomass, is a complex aqueous product that is difficult to utilize and usually discarded. This work presents the feasibility of light oil as a sole carbon source to support the growth of Rhodococcus opacus (R. opacus) that in turn accumulate triacylglycerols as biodiesel feedstock. Two types of bacteria (R. opacus PD630 and DSM 1069) were selected in this study. Research results showed that after short adaption periods both strains can grow well on this complex carbon source, as proved by the consumption of oligomers and monomers in light oil. Lipid content by R. opacus PD630 and DSM 1069 was observed up to 25.8 % and 22.0 % of cell dry weight, respectively. Palmitic and stearic acids were found to be the predominant fatty acids in these bacterial cells. In addition, the light oil-based lipid production can be enhanced by reducing the pH value from 7 to 4, especially in case of DSM 1069.
热解产生的轻质油占起始生物质碳产率的10%左右，是一种难以利用且通常被丢弃的复杂水性产物。这项工作展示了轻质油作为唯一碳源来支持不透明红球菌（R. opacus）生长的可行性，而不透明红球菌反过来会积累三酰甘油作为生物柴油原料。本研究选择了两种细菌（不透明红球菌PD630和DSM 1069）。研究结果表明，经过短暂的适应期后，两种菌株都能在这种复杂碳源上良好生长，轻质油中低聚物和单体的消耗证明了这一点。观察到不透明红球菌PD630和DSM 1069的脂质含量分别高达细胞干重的25.8%和22.0%。棕榈酸和硬脂酸是这些细菌细胞中的主要脂肪酸。此外，通过将pH值从7降低到4可以提高基于轻质油的脂质产量，尤其是对于DSM 1069而言。
