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基于 iTRAQ 的槲寄生多糖对 HepG2 细胞抑制作用的定量蛋白质组学分析。

iTRAQ-Based Quantitative Proteomic Analysis of the Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells.

机构信息

Northeast Forestry University, Harbin, PR China.

出版信息

Sci Rep. 2017 Jul 4;7(1):4596. doi: 10.1038/s41598-017-04417-x.

DOI:10.1038/s41598-017-04417-x
PMID:28676664
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5496916/
Abstract

Viscum coloratum (Kom.) Nakai is one of active medicinal plants, and its active components, especially polysaccharides, have been shown to exhibit bioactivity. In this study, we examined the effects of three polysaccharide fractions from Viscum coloratum (Kom.) Nakai on HepG2 cell growth in a dose-dependent manner by using a CCK-8 assay kit. Flow cytometry analysis showed that VCP2 treatment delayed the cell cycle in the G1 phase and induced apoptosis in HepG2 cells, a result possibly due to the increased expression of p21 and Cyclin D and the decreased expression of Cyclin E and CDK4. The increased expression of Bad, Smac and Caspase-3 and the decreased expression of Bcl-XL and XIAP may be some of the reasons for the induction of apoptosis in VCP2-treated HepG2 cells. Through iTRAQ and 2D-LC-MSMS, 113 and 198 differentially expressed proteins were identified in normal and VCP2-treated HepG2 and Caco2 cells. The mRNA and protein levels of Histone H3.1, Cytoskeletal 9 and Vitronectin agreed with iTRAQ proteomic results. GO, pathways and the PPI of differentially expressed proteins were further analyzed. These findings broaden the understanding of the anti-tumor mechanisms of mistletoe polysaccharides and provide new clues for screening proteins that are responsive to polysaccharides.

摘要

槲寄生（Viscum coloratum）是一种具有生物活性的药用植物，其活性成分，尤其是多糖，已被证明具有生物活性。在这项研究中，我们使用 CCK-8 试剂盒，以剂量依赖的方式，检测了槲寄生三种多糖级分（Viscum coloratum）对 HepG2 细胞生长的影响。流式细胞术分析表明，VCP2 处理使 HepG2 细胞周期阻滞于 G1 期并诱导细胞凋亡，这可能是由于 p21 和 Cyclin D 的表达增加以及 Cyclin E 和 CDK4 的表达减少所致。Bad、Smac 和 Caspase-3 的表达增加以及 Bcl-XL 和 XIAP 的表达减少可能是 VCP2 诱导 HepG2 细胞凋亡的部分原因。通过 iTRAQ 和 2D-LC-MSMS，在正常和 VCP2 处理的 HepG2 和 Caco2 细胞中鉴定到 113 个和 198 个差异表达蛋白。Histone H3.1、Cytoskeletal 9 和 Vitronectin 的 mRNA 和蛋白水平与 iTRAQ 蛋白质组学结果一致。进一步分析了差异表达蛋白的 GO、通路和 PPI。这些发现拓宽了对槲寄生多糖抗肿瘤机制的认识，并为筛选对多糖有反应的蛋白质提供了新的线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/446bca0dfdc8/41598_2017_4417_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/f4739c14b5c9/41598_2017_4417_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/78ad9ddcf134/41598_2017_4417_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/88e91a7ac984/41598_2017_4417_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/a68dc98e2b16/41598_2017_4417_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/cb9864bcc008/41598_2017_4417_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/446bca0dfdc8/41598_2017_4417_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/f4739c14b5c9/41598_2017_4417_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/78ad9ddcf134/41598_2017_4417_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/88e91a7ac984/41598_2017_4417_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/a68dc98e2b16/41598_2017_4417_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/cb9864bcc008/41598_2017_4417_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5b6/5496916/446bca0dfdc8/41598_2017_4417_Fig6_HTML.jpg

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4
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