School of Forestry, Northeast Forestry University, Harbin 150040, PR China.
College of Life Sciences, Northeast Forestry University, Harbin 150040, PR China; Northeast Petroleum University, Daqing 163318, PR China.
Int J Biol Macromol. 2019 Aug 1;134:588-594. doi: 10.1016/j.ijbiomac.2019.04.173. Epub 2019 Apr 25.
An enzyme-assisted extraction method had been optimized by single factor experiments and orthogonal test design, to improve the extract yield of polysaccharides from the leaves of Viscum coloratum (Kom.) Nakai (VCP). The anti-HBV activity of VCP in vitro was investigated by PCR-fluorescent probing (FQ-PCR) and ELISA methods. Moreover the antioxidant activity of VCP in vitro was studied in terms of the reducing power, 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) test radical-scavenging activity and hydroxyl radicals-scavenging activity. The results demonstrated that the optimum extraction conditions were solid-liquid ratio of 1:40, enzyme concentrations of 2.5%, enzyme action time of 40 min, enzyme action temperature of 50 °C, enzymatic pH of 5. Under these conditions, the extraction yield of VCP was 21.83 ± 0.45%. VCP could inhibit the replication of HBV-DNA and the secretion of HBV antigens in a dose-dependent manner, and showed better antioxidant capacity. These findings suggest that VCP could be a good potential natural antiviral agent and antioxidant.
采用单因素实验和正交试验设计优化了酶辅助提取方法，以提高槲寄生（Viscum coloratum（Kom.）Nakai）叶多糖的提取产率。通过 PCR-荧光探针（FQ-PCR）和 ELISA 方法研究了 VCP 的体外抗 HBV 活性。此外，还从还原能力、1,1-二苯基-2-苦基肼（DPPH）自由基清除活性和羟基自由基清除活性等方面研究了 VCP 的体外抗氧化活性。结果表明，最佳提取条件为固液比 1:40、酶浓度 2.5%、酶作用时间 40min、酶作用温度 50°C、酶作用 pH 值 5。在此条件下，VCP 的提取率为 21.83±0.45%。VCP 可以剂量依赖性地抑制 HBV-DNA 的复制和 HBV 抗原的分泌，并表现出更好的抗氧化能力。这些发现表明，VCP 可能是一种良好的天然抗病毒和抗氧化剂。
