Pierini Alessio, Millanta Francesca, Zanforlin Roberta, Vannozzi Iacopo, Marchetti Veronica
Veterinary Teaching Hospital, Department of Veterinary Science, University of Pisa, Italy.
J Vet Diagn Invest. 2017 Nov;29(6):869-873. doi: 10.1177/1040638717718886. Epub 2017 Jul 5.
We determined cytologic features of histologically confirmed subcentimeter canine mammary tumors (CMTs) to determine reasonable criteria for an accurate cytologic diagnosis. Fifty-three CMTs from 28 bitches were included. All cytologic samples were collected by ultrasound-guided fine-needle aspiration biopsy, stained with May-Grünwald/Giemsa, and retrospectively evaluated using a scoring system established for 18 cytologic features. Mean nuclear area (MNA) was also measured for each sample by a computer-assisted program. Based on the histologic diagnosis, CMTs were divided into 2 groups: malignant tumors (25) and benign lesions (15). Data were statistically analyzed using Fisher and Mann-Whitney tests. Chromatin pattern ( p < 0.05) and macrophage infiltration ( p < 0.05) were significantly different between the groups. Median MNA was significantly ( p < 0.05) larger in malignant tumors. The evaluation of these cytologic features in subcentimeter CMTs may increase the sensitivity of cytology.
我们确定了经组织学证实的亚厘米级犬乳腺肿瘤（CMTs）的细胞学特征，以确定准确细胞学诊断的合理标准。纳入了来自28只母犬的53个CMTs。所有细胞学样本均通过超声引导下细针穿刺活检采集，用May-Grünwald/Giemsa染色，并使用针对18个细胞学特征建立的评分系统进行回顾性评估。还通过计算机辅助程序测量每个样本的平均核面积（MNA）。根据组织学诊断，CMTs分为两组：恶性肿瘤（25个）和良性病变（15个）。使用Fisher检验和Mann-Whitney检验对数据进行统计学分析。两组之间的染色质模式（p < 0.05）和巨噬细胞浸润（p < 0.05）有显著差异。恶性肿瘤的MNA中位数显著更大（p < 0.05）。对亚厘米级CMTs的这些细胞学特征进行评估可能会提高细胞学检查的敏感性。
