使用非整合系统从健康供体的尿液细胞中生成人诱导多能干细胞。
Generation of human induced pluripotent stem cells from urinary cells of a healthy donor using a non-integration system.
作者信息
Uhm Kyung-Ok, Jo Eun Hee, Go Gue Youn, Kim So-Jung, Choi Hye Young, Im Young Sam, Ha Hye-Yeong, Jung Ji-Won, Koo Soo Kyung
机构信息
Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
Division of Intractable Disease, Center for Biomedical Sciences, Korea National Institute of Health, Chungcheongbuk-do, South Korea.
出版信息
Stem Cell Res. 2017 May;21:44-46. doi: 10.1016/j.scr.2017.03.019. Epub 2017 Mar 30.
Urinary cells can be an ideal source for generating hiPSCs and progenitors, as they are easily accessible, non-invasive, and universally available. We generated human induced pluripotent stem cells (hiPSCs) from the urinary cells of a healthy donor using a Sendai virus-based gene delivery method. The generated hiPSC line, KSCBi001-A, has a normal karyotype (46,XY). The pluripotency and capacity of multilineage differentiation were characterized by comparison with those of a human embryonic stem cell line. This cell line is registered and available from National Stem Cell Bank, Korea National Institute of Health.
尿源细胞可以成为生成人诱导多能干细胞（hiPSC）及其祖细胞的理想来源，因为它们易于获取、非侵入性且普遍可得。我们使用基于仙台病毒的基因递送方法，从一名健康供体的尿源细胞中生成了人诱导多能干细胞（hiPSC）。所生成的hiPSC系KSCBi001-A具有正常的核型（46,XY）。通过与人类胚胎干细胞系进行比较，对其多能性和多谱系分化能力进行了表征。该细胞系已在韩国国立卫生研究院国家干细胞库注册并可供使用。
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