Kamei Hiroyasu, Duan Cunming
Noto Marine Laboratory, Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Noto, Ishikawa, Japan.
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Methods Mol Biol. 2018;1742:195-203. doi: 10.1007/978-1-4939-7665-2_17.
Zebrafish has emerged as an informative animal model to study the biological impact and molecular mechanisms of hypoxia. Here we describe a simple method to induce hypoxia in zebrafish embryos and larvae. This protocol is easy and reproducible and does not require expensive equipment or specialized devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and can be applied to other fish and amphibian species.
斑马鱼已成为研究缺氧的生物学影响和分子机制的一种信息丰富的动物模型。在此，我们描述一种在斑马鱼胚胎和幼体中诱导缺氧的简单方法。该方案简便且可重复，不需要昂贵的设备或专门的装置。它可在大、中、小尺度上进行调整。该方案也非常适合需要化学药物处理的实验，并且可应用于其他鱼类和两栖类物种。
