用于斑马鱼基因组编辑和功能研究的显微注射

Microinjection in Zebrafish for Genome Editing and Functional Studies.

作者信息

Pei Wuhong, Burgess Shawn M

机构信息

Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

出版信息

Methods Mol Biol. 2019;1874:459-474. doi: 10.1007/978-1-4939-8831-0_26.

DOI:10.1007/978-1-4939-8831-0_26

PMID:30353530

Abstract

A major strength of zebrafish as a model organism is their rapid, in vitro development. The easy access to embryos compared to mammals, allows larval molecular and cellular composition to be manipulated by microinjection, providing a powerful avenue for biological and translational studies. Here, we describe the essential steps and different applications of microinjection in zebrafish for genome editing and functional studies, along with some experimental tips that are critical for microinjection success.

摘要

斑马鱼作为一种模式生物的一个主要优势在于其快速的体外发育。与哺乳动物相比，获取斑马鱼胚胎更容易，这使得可以通过显微注射来操控幼体的分子和细胞组成，为生物学研究和转化研究提供了一条有力途径。在此，我们描述了在斑马鱼中进行显微注射以用于基因组编辑和功能研究的基本步骤、不同应用，以及一些对显微注射成功至关重要的实验技巧。