Gardizani Taiane Priscila, Pinke Karen Henriette, Lima Heliton Gustavo de, Lara Vanessa Soares
Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Cirurgia, Estomatologia, Patologia e Radiologia. Bauru, SP, Brasil.
J Appl Oral Sci. 2017 May-Jun;25(3):265-273. doi: 10.1590/1678-7757-2016-0322.
The aim of this study was to investigate the influence of aging on the internalization and the production of nitric oxide (NO) by peritoneal adherent cells (PAC), in response to Candida albicans (C. albicans).
PAC obtained from young and aged mice were challenged with dead or viable C. albicans by using predetermined proportions (cells:yeast) for 30 and 120 minutes. Phagocytosis was analyzed by acridine orange dye, and NO production by the Griess reaction.
C. albicans phagocytosis by PAC from aged mice was similar to that of young mice, although the cells from older mice cells present more internalized fungi compared with matched control. In addition, a tendency towards impaired NO production by peritoneal mononuclear phagocytes from aged mice was observed.
PAC from aged mice may capture and store many fungi, which in turn may mean that these cells are effectively unable to eliminate fungi, probably due to impaired NO production. Therefore, considering the important role of C. albicans overgrowth in the pathogenesis of DS and the aspects observed in this study, aging may favor the onset and severity of local candidosis such as DS and its systemic forms.
本研究旨在探讨衰老对腹膜黏附细胞（PAC）内化白色念珠菌（白色念珠菌）及产生一氧化氮（NO）的影响。
使用预定比例（细胞：酵母），用死的或活的白色念珠菌对从年轻和老年小鼠获得的PAC进行30分钟和120分钟的刺激。通过吖啶橙染料分析吞噬作用，通过格里斯反应分析NO的产生。
老年小鼠的PAC对白色念珠菌的吞噬作用与年轻小鼠相似，尽管与匹配的对照相比，老年小鼠细胞内化的真菌更多。此外，观察到老年小鼠腹膜单核吞噬细胞产生NO的能力有受损的趋势。
老年小鼠的PAC可能捕获并储存许多真菌，这反过来可能意味着这些细胞实际上无法有效清除真菌，这可能是由于NO产生受损所致。因此，考虑到白色念珠菌过度生长在DS发病机制中的重要作用以及本研究中观察到的方面，衰老可能有利于局部念珠菌病（如DS及其全身形式）的发生和严重程度。
