Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Gerodontology. 2012 Jun;29(2):e331-9. doi: 10.1111/j.1741-2358.2011.00476.x. Epub 2011 Apr 1.
Elderly individuals with Candida-related denture stomatitis (DS) present with a reduced defence against Candida albicans. This study evaluated levels of antimicrobial mediators in the elderly DS saliva and salivary neutrophils' activation characteristics compared with elderly and young without DS.
Salivary peroxidases (SPO) and elastase activities (ELA), nitric oxide (NO), transforming growth factor beta (TGF-β), IL-6 and CCL3 production were determined in saliva from elderly with or without DS, and young control individuals. TLR4, CXCR1, CD11b, CD16 and CD32 expression on salivary neutrophils were evaluated. Correlations between number and apoptosis rate of salivary neutrophils, enzymatic activities and cytokine levels were determined.
Elderly DS individuals exhibited the lowest SPO and ELA activities. Also, the activity of both enzymes was low in elderly without DS. Although both elderly groups showed higher salivary NO and TGF-β levels compared to young control groups, elderly DS presented the highest salivary NO, TGF-β, IL-6 and CCL3 levels. Decreased percentages of salivary TLR4(+) and CD16(+) neutrophils were detected in both elderly groups. Although these damages could influence the establishment and persistence of DS, the highest levels of salivary IL-6 and CCL3 in elderly DS could be preventing more serious complications.
患有念珠菌相关性义齿性口炎（DS）的老年人对白色念珠菌的防御能力降低。本研究评估了与无 DS 的老年和年轻个体相比，老年 DS 唾液中的抗菌介质水平和唾液中性粒细胞的激活特征。
测定了有或无 DS 的老年患者以及年轻对照组个体唾液中的过氧化物酶（SPO）和弹性蛋白酶（ELA）活性、一氧化氮（NO）、转化生长因子β（TGF-β）、IL-6 和 CCL3 的产生。评估了唾液中性粒细胞上 TLR4、CXCR1、CD11b、CD16 和 CD32 的表达。确定了唾液中性粒细胞数量和凋亡率、酶活性和细胞因子水平之间的相关性。
老年 DS 个体的 SPO 和 ELA 活性最低。而且，无 DS 的老年个体的这两种酶的活性也较低。尽管两组老年个体的唾液 NO 和 TGF-β水平均高于年轻对照组，但老年 DS 组的唾液 NO、TGF-β、IL-6 和 CCL3 水平最高。在两组老年个体中，均检测到唾液 TLR4（+）和 CD16（+）中性粒细胞的百分比降低。尽管这些损伤可能会影响 DS 的建立和持续存在，但老年 DS 中最高水平的唾液 IL-6 和 CCL3 可能会防止更严重的并发症。
