Valentim Diego, Bueno Carlos Roberto Emerenciano, Marques Vanessa Abreu Sanches, Vasques Ana Maria Veiga, Cury Marina Tolomei Sandoval, Cintra Luciano Tavares Angelo, Dezan Eloi
Centro Universitário do Distrito Federal - UDF, School of Dentistry, Department of Endodontics, Brasilia, DF, Brazil.
Universidade Estadual Paulista - UNESP, Araçatuba School of Dentistry, Department of Endodontics, Araçatuba, SP, Brazil.
Braz Oral Res. 2017 Jul 3;31:e43. doi: 10.1590/1807-3107BOR-2017.vol31.0043.
The aim of this study was to evaluate edemogenic activity and subcutaneous inflammatory reaction induced by Psidium cattleianum leaf extracts associated with Ca(OH)2. Thirty male Wistar rats, split equally into three groups [aqueous extract + Ca(OH)2; ethanolic extract + Ca(OH)2; and propylene glycol + Ca(OH)2], were assessed every 3 h or 6 h (five animals in each period). Under general anesthesia, 0.2 mL of 1% Evans blue per 100 g of body weight was injected into the penile vein and each combination to be evaluated was subcutaneously injected into the dorsal region 30 min thereafter. Edemogenic activity was analyzed by spectrophotometry (λ=630 nm). For inflammatory reaction analysis, 50 rats received four polyethylene tubes (three experimental groups) and an empty tube (control group). The assessments were made at 7, 15, 30, 60, and 90 days, followed by hematoxylin-eosin staining and by the assignment of scores for evaluation of tissue response intensity. Ethanolic extract + Ca(OH)2 yielded the largest edemogenic activity at 3 h. Intergroup differences at 6 h were not significant. The histological analysis showed progressive repair over time (p<0.05) and aqueous and ethanolic extracts produced similar responses to those of the control and Ca(OH)2 + propylene glycol groups. Psidium cattleianum leaf extracts used as Ca(OH)2 vehicles evoked similar tissue response when compared to Ca(OH)2 associated with propylene glycol.
本研究的目的是评估番樱桃叶提取物与氢氧化钙联合使用时的致水肿活性和皮下炎症反应。将30只雄性Wistar大鼠平均分为三组[水提取物+氢氧化钙；乙醇提取物+氢氧化钙；丙二醇+氢氧化钙]，每3小时或6小时评估一次（每个时间段5只动物）。在全身麻醉下，每100克体重经阴茎静脉注射0.2毫升1%伊文思蓝，30分钟后将每种待评估的组合物皮下注射到背部区域。通过分光光度法（λ=630nm）分析致水肿活性。为了分析炎症反应，50只大鼠接受了四根聚乙烯管（三个实验组）和一根空管（对照组）。在第7、15、30、60和90天进行评估，随后进行苏木精-伊红染色，并为组织反应强度评估打分。乙醇提取物+氢氧化钙在3小时时产生的致水肿活性最大。6小时时组间差异不显著。组织学分析显示随时间推移有进行性修复（p<0.05），水提取物和乙醇提取物产生的反应与对照组和氢氧化钙+丙二醇组相似。与丙二醇联合使用的氢氧化钙相比，用作氢氧化钙载体的番樱桃叶提取物引起的组织反应相似。
