Department of Restorative Dentistry, Endodontics, Araçatuba School of Dentistry, UNESP Univ Estadual Paulista, Rua José Bonifácio, 1193, Araçatuba, SP, 16015-050, Brazil.
Department of Pathology, Microbiology and Imunology, Araçatuba School of Dentistry, UNESP Univ Estadual Paulista, São Paulo, Brazil.
Clin Oral Investig. 2018 Jul;22(6):2273-2279. doi: 10.1007/s00784-017-2326-5. Epub 2018 Jan 9.
Evaluate, in vitro, the antimicrobial activity of Psidium cattleianum leaf extracts combined with calcium hydroxide against Enterococcus faecalis and Candida albicans biofilm.
Dentin specimens obtained from extracted bovine incisors were infected during 14 days with E. faecalis ATCC 29212 and C. albicans ATCC 10231. The specimens were filled with calcium hydroxide pastes prepared with the following vehicles: Psidium cattleianum ethanolic, Psidium cattleianum propylene glycolic, distilled water, and saline as control. After 24 h, 3, 7, and 14 days, the canals were irrigated with sterile saline and dried. Dentin samples were collected from the canals with burs of increasing diameters. To determine the number of colony-forming units (CFU), samples were inoculated onto BHI agar supplemented with yeast extract (0.5%), at 37 °C, for 48 h, in CO enriched atmosphere. Comparisons among the groups for the variation factors were performed by ANOVA and Tukey's test.
Ethanolic and propylene glycolic extracts showed significantly higher antimicrobial activity against E. faecalis (p < 0.01) when compared with distilled water. The ethanolic extract exhibited in 24 h the same antibacterial activity that propylene glycolic extract and distilled water after 7 and 14 days. For C. albicans, all were effective in reducing the number of CFU at all periods.
The P. cattleianum ethanolic extract presented the fastest and highest antimicrobial activity against E. faecalis, significantly reducing the microbial load in 24 h. All medications were effective against C. albicans.
The antibacterial potential of P. cattleianum and its biological compatibility associated with calcium hydroxide indicate promising applications in the field of dentistry.
体外评估番樱桃叶提取物与氢氧化钙联合应用对粪肠球菌和白色念珠菌生物膜的抗菌活性。
从拔除的牛门牙中获得牙本质标本，用粪肠球菌 ATCC 29212 和白色念珠菌 ATCC 10231 感染 14 天。标本用以下载剂制备的氢氧化钙糊剂填充：番樱桃叶乙醇、番樱桃叶丙二醇、蒸馏水和生理盐水作为对照。24 小时后、第 3、7 和 14 天，用无菌生理盐水冲洗管腔并干燥。用直径逐渐增大的车针从管腔中收集牙本质样本。为了确定菌落形成单位（CFU）的数量，将样本接种到补充有酵母提取物（0.5%）的 BHI 琼脂上，在 37°C 下，在 CO 富集的气氛中培养 48 小时。通过方差分析和 Tukey 检验对各组的变异因素进行比较。
与蒸馏水相比，乙醇和丙二醇提取物对粪肠球菌的抗菌活性明显更高（p<0.01）。乙醇提取物在 24 小时时表现出与丙二醇提取物和蒸馏水相同的抗菌活性，而在 7 天和 14 天后则表现出相同的抗菌活性。对于白色念珠菌，所有药物在所有时期都能有效降低 CFU 的数量。
番樱桃叶乙醇提取物对粪肠球菌表现出最快和最高的抗菌活性，在 24 小时内显著降低了微生物负荷。所有药物对白色念珠菌均有效。
番樱桃叶的抗菌潜力及其与氢氧化钙的生物相容性表明其在牙科领域有应用前景。
