Gerhard Dayana, Sousa Frederico José da Silva Simão de, Andraus Rodrigo Antonio Carvalho, Pardo Paulo Eduardo, Nai Gisele Alborguetti, Neto Hermann Bremer, Messora Michel Reis, Maia Luciana Prado
Universidade do Oeste Paulista - Unoeste, School of Dentistry, Presidente Prudente, SP, Brazil.
Universidade Norte do Paraná - Unopar, Rehabilitation Sciences Program, Londrina, PR, Brazil.
Braz Oral Res. 2017 Jul 3;31:e71. doi: 10.1590/1807-3107BOR-2017.vol31.0071.
The aim of the present study was to evaluate the effect of systemic administration of probiotics (PROB) on the progression of experimentally induced oral and intestinal mucositis in rats immunosuppressed by chemotherapy (5-fluorouracil: 5-FU). Twenty-four rats were divided into the following groups (n=6): GC (control), GPROB, G5FU and G5-FU/PROB. Groups GPROB and G5-FU/PROB received 1 g of probiotic incorporated into each 100 g of feed (Bacillus subtilis, Bifidobacterium bifidum, Enterococcus faecium and Lactobacilllus acidophilus), beginning 30 days before oral mucositis induction. Groups G5FU and G5-FU/PROB received 60 mg/kg of 5-FU on days 0 and 2. The left oral mucosa of each animal was irritated by mechanical trauma (days 1 and 2). On days 3 and 7, three animals from each group were sacrificed, and their oral mucosa and small intestine were biopsied and processed for histopathological analysis. Groups G5-FU and G5-FU/PROB showed ulcerated oral lesions at day 3, with progression in group G5-FU and regression in group G5-FU/PROB at day 7. Histologically, less severe signs of inflammation in the oral mucosa were observed in group G5-FU/PROB than in group G5-FU. Regarding the intestine, villus-related defects of lesser magnitude were observed in group G5-FU/PROB, compared with group G5-FU. Group GPROB showed greater villus height than group GC. It can be concluded that probiotic supplementation reduced oral and intestinal inflammation in immunosuppressed rats with experimentally induced mucositis, and may protect the intestine from changes induced by chemotherapy, thus contributing to overall health.
本研究的目的是评估全身给予益生菌（PROB）对化疗（5-氟尿嘧啶：5-FU）免疫抑制大鼠实验性诱导的口腔和肠道粘膜炎进展的影响。24只大鼠分为以下几组（n = 6）：GC组（对照组）、GPROB组、G5FU组和G5-FU/PROB组。GPROB组和G5-FU/PROB组在口腔粘膜炎诱导前30天开始，每100克饲料中添加1克益生菌（枯草芽孢杆菌、双歧双歧杆菌、粪肠球菌和嗜酸乳杆菌）。G5FU组和G5-FU/PROB组在第0天和第2天接受60 mg/kg的5-FU。每只动物的左侧口腔粘膜通过机械创伤进行刺激（第1天和第2天）。在第3天和第7天，每组处死3只动物，对其口腔粘膜和小肠进行活检并进行组织病理学分析。G5-FU组和G5-FU/PROB组在第3天出现口腔溃疡病变，G5-FU组在第7天病变进展，而G5-FU/PROB组病变消退。组织学上，G5-FU/PROB组口腔粘膜炎症迹象比G5-FU组轻。在肠道方面，与G5-FU组相比，G5-FU/PROB组观察到绒毛相关缺陷程度较轻。GPROB组的绒毛高度高于GC组。可以得出结论，补充益生菌可减轻实验性诱导粘膜炎的免疫抑制大鼠的口腔和肠道炎症，并可能保护肠道免受化疗引起的变化，从而有助于整体健康。
