Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
J Periodontol. 2013 Dec;84(12):1818-26. doi: 10.1902/jop.2013.120644. Epub 2013 Jan 17.
With increase in the incidence of resistance to antibiotics, probiotics are emerging as a promising adjunctive periodontal therapy. The authors of this study evaluate the influence of probiotic (PROB) supplementation on ligature-induced periodontitis (LIP) and intestinal morphology in rats.
Thirty-two rats were randomly divided into four groups: control (C), LIP, PROB, and LIP/PROB. In groups PROB and LIP/PROB, the PROB was administered orally by addition to the drinking water of the animals for 44 days. In groups LIP and LIP/PROB, the mandibular right first molar of the animals received a cotton ligature that was left in the same position for 14 days. All animals were euthanized 44 days after the start of the PROB supplementation. The jaws were resected and histomorphometric analyses were performed. The measurements included evaluation of attachment loss (AL) and alveolar bone level (ABL) on the distal root of the mandibular first molar. Samples of the duodenum, jejunum, and ileum were also dissected from each animal to evaluate the villous height (VH) and crypt depth (CD). The data obtained were subjected to statistical analyses (analysis of variance, Tukey; P <0.05).
Mean values of AL and ABL were significantly higher in group LIP compared with group LIP/PROB (AL: 3.05 ± 0.57 mm and 1.78 ± 0.63 mm, respectively; ABL: 4.21 ± 0.42 mm and 3.38 ± 0.17 mm, respectively). In group LIP/PROB, the mean values of VH and CD of the jejunum were significantly higher than the ones from group LIP (VH: 672.1 ± 83.3 µm and 528.0 ± 51.7 µm, respectively; CD: 463.8 ± 100.9 µm and 269.0 ± 48.4 µm, respectively).
It can be concluded that PROB supplementation 1) reduces AL and alveolar bone loss in rats with LIP and 2) can protect the small intestine from reactive changes induced by LIP.
随着抗生素耐药性的增加，益生菌作为一种有前途的牙周辅助治疗方法正在出现。本文作者评估了益生菌（PROB）补充对结扎诱导的牙周炎（LIP）和大鼠肠道形态的影响。
32 只大鼠随机分为四组：对照组（C）、LIP 组、PROB 组和 LIP/PROB 组。在 PROB 组和 LIP/PROB 组中，通过在动物饮用水中添加 PROB 进行口服给药，持续 44 天。在 LIP 组和 LIP/PROB 组中，动物右侧下颌第一磨牙用棉线结扎，14 天内保持在同一位置。所有动物在 PROB 补充开始后 44 天被安乐死。切除颌骨并进行组织形态计量学分析。测量包括下颌第一磨牙远中根的附着丧失（AL）和牙槽骨水平（ABL）。还从每个动物中解剖出十二指肠、空肠和回肠样本，以评估绒毛高度（VH）和隐窝深度（CD）。获得的数据进行了统计分析（方差分析，Tukey；P<0.05）。
与 LIP/PROB 组相比，LIP 组的 AL 和 ABL 平均值显着升高（AL：3.05±0.57mm 和 1.78±0.63mm；ABL：4.21±0.42mm 和 3.38±0.17mm）。在 LIP/PROB 组中，空肠的 VH 和 CD 平均值显着高于 LIP 组（VH：672.1±83.3μm 和 528.0±51.7μm；CD：463.8±100.9μm 和 269.0±48.4μm）。
可以得出结论，PROB 补充 1）减少了 LIP 大鼠的 AL 和牙槽骨丢失，2）可以保护小肠免受 LIP 引起的反应性变化。
