Inoue H, Seyama Y, Yamashita S
Experientia. 1986 Jan 15;42(1):33-5. doi: 10.1007/BF01975881.
Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was quantitatively determined using HPLC equipped with an amperometric detector. The method was used to analyze the activity of AS-A in biological materials.
生物材料中的芳基硫酸酯酶活性太低，无法用迄今为止可用的方法检测到。本研究开发了一种灵敏且特异的芳基硫酸酯酶A（AS-A）检测方法。已知抗坏血酸-2-硫酸盐是AS-A的特异性天然底物；使用配备安培检测器的高效液相色谱法定量测定AS-A作用释放的抗坏血酸。该方法用于分析生物材料中AS-A的活性。
