一种用于同时和分别测定芳基硫酸酯酶A和B的灵敏荧光测定法。
A sensitive fluorescence assay for the simultaneous and separate determination of arylsulphatases A and B.
作者信息
Christomanou H, Sandhoff K
出版信息
Clin Chim Acta. 1977 Sep 15;79(3):527-31. doi: 10.1016/0009-8981(77)90172-3.
PMID:19184
Abstract
A sensitive fluorometric assay utilizing 4-methylumbelliferyl sulphate has been developed for the simultaneous determination of arylsulphatases A and B from leucocytes, based on the differential effect of silver ions on the two enzymes. The procedure allows discrimination between normal cases and those with metachromatic leucodystrophy.
摘要
基于银离子对两种酶的不同作用，开发了一种利用4-甲基伞形酮基硫酸盐的灵敏荧光测定法，用于同时测定白细胞中的芳基硫酸酯酶A和B。该方法能够区分正常病例和患有异染性脑白质营养不良的病例。
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