WAP四二硫键核心结构域蛋白2通过调控转移相关基因促进人卵巢癌转移。

WAP four-disulfide core domain protein 2 promotes metastasis of human ovarian cancer by regulation of metastasis-associated genes.

作者信息

Chen Yao, Huang Liping, Wang Suihai, Liu Tiancai, Wu Yingsong, Li Ji-Liang, Li Ming

机构信息

School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510515, China.

State Key Laboratory of Organ Failure, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou, 510515, China.

出版信息

J Ovarian Res. 2017 Jul 5;10(1):40. doi: 10.1186/s13048-017-0329-0.

BACKGROUND

WAP four-disulfide core domain protein 2 (WFDC2) shows a tumor-restricted upregulated pattern of expression in ovarian cancer.

METHODS

In this study, we evaluated the role of WFCD2 in tumor mobility, invasion and metastasis of ovarian cancer in clinical tissue and in ovarian cancer cells, both in vitro and in vivo.

RESULTS

Our results revealed WFCD2 was overexpressed in ovarian tissues, and the expression level of WFCD2 was associated with metastasis and lymph node metastasis. Higher expression of WFCD2 was also observed in aggressive HO8910-PM cells than in HO8910 cells, and WFCD2 knockdown halted cell migration, invasion, tumorigenicity and metastasis in ovarian cancer cells, both in vitro and in vivo. Knockdown of WFDC2 induced the down-regulation of ICAM-1, CD44, and MMP2.

CONCLUSION

In summary, our work demonstrates that WFCD2 promotes metastasis in ovarian cancer. These findings suggest that WFCD2 plays a critical role in promoting metastasis and may constitute a potential therapeutic target of ovarian cancer.

背景

富含半胱氨酸的分泌蛋白2（WFDC2）在卵巢癌中呈现肿瘤特异性上调的表达模式。

方法

在本研究中，我们评估了WFCD2在临床组织以及体外和体内卵巢癌细胞中对卵巢癌肿瘤移动性、侵袭和转移的作用。

结果

我们的结果显示WFCD2在卵巢组织中过表达，且WFCD2的表达水平与转移及淋巴结转移相关。在侵袭性HO8910 - PM细胞中也观察到WFCD2的表达高于HO8910细胞，并且敲低WFCD2可在体外和体内抑制卵巢癌细胞的迁移、侵袭、致瘤性和转移。敲低WFDC2可诱导细胞间黏附分子-1（ICAM - 1）、CD44和基质金属蛋白酶2（MMP2）的下调。