Department of Gynecology, People's Hospital of Binzhou, Binzhou, Shandong 256610, P.R. China.
The Seventh People's Hospital of Jinan, Jiyang, Shandong 251400, P.R. China.
Mol Med Rep. 2017 Oct;16(4):5151-5156. doi: 10.3892/mmr.2017.7286. Epub 2017 Aug 18.
Mutations of A‑kinase anchoring protein 2 (AKAP2) have been reported to be associated with adolescent idiopathic scoliosis. However, its role in cancer is poorly understood. In the present study, the mRNA levels of AKAP2 in ovarian cancer tissues were examined using qPCR. The effects of AKAP2 on the growth and migration of cancer cells were examined using crystal violet and Boyden chamber assays. An in vivo image system was used to evaluate the effect of AKAP2 on the metastasis of ovarian cancer cells. The present study demonstrated that the expression of AKAP2 was elevated in ovarian cancer. Furthermore, overexpression of AKAP2 promoted the growth and migration of ovarian cancer cells, whereas knockdown of AKAP2 expression reduced the growth and migration of ovarian cancer cells. Analysis of the molecular mechanism indicated that AKAP2 activated β‑catenin/T cell factor signaling and regulated the expression of several target genes. Furthermore, analysis of the in vivo metastatic capacity demonstrated that downregulation of AKAP2 inhibited the invasion of ovarian cancer cells. Taken together, the present study demonstrated an oncogenic role for AKAP2 in ovarian cancer, indicating that AKAP2 may be a therapeutic target for ovarian cancer.
AKAP2 的突变与青少年特发性脊柱侧凸有关。然而，其在癌症中的作用尚不清楚。在本研究中，通过 qPCR 检测了卵巢癌组织中 AKAP2 的 mRNA 水平。通过结晶紫和 Boyden 室分析检测 AKAP2 对癌细胞生长和迁移的影响。利用体内成像系统评估 AKAP2 对卵巢癌细胞转移的影响。本研究表明，AKAP2 在卵巢癌中表达上调。此外，过表达 AKAP2 促进了卵巢癌细胞的生长和迁移，而敲低 AKAP2 表达则降低了卵巢癌细胞的生长和迁移。分子机制分析表明，AKAP2 激活了 β-连环蛋白/T 细胞因子信号通路，并调节了几个靶基因的表达。此外，体内转移能力分析表明，下调 AKAP2 抑制了卵巢癌细胞的侵袭。综上所述，本研究表明 AKAP2 在卵巢癌中具有致癌作用，表明 AKAP2 可能是卵巢癌的治疗靶点。
