Department of Gynecologic Oncology, Women's Hospital School of Medicine Zhejiang University, No. 1 Xueshi Road, Hangzhou, 310006, China.
Women's Reproductive Health Research Laboratory of Zhejiang Province, Women's Hospital School of Medicine Zhejiang University, Hangzhou, China.
J Ovarian Res. 2021 Feb 24;14(1):38. doi: 10.1186/s13048-021-00776-2.
To investigate the detailed roles and mechanisms of tumor-derived exosomes in progression and metastasis of ovarian cancer in vitro.
Exosomes were isolated by differential centrifugation method; the morphology, size and biological markers of exosomes were separately defined by transmission electron microscopy, nanoS90 and Western blotting; Trans-well chambers assay was used to assess the ability of migration and invasion of recipient cells uptaking the exosomes from HO8910PM cells. The downstream molecule was screened by mass spectrometry.CD44 was identified by western blotting and the function of CD44 was identified by trans-well chambers assay and CCK8 assay.
Exosomes derived from HO8910PM cells could be transferred to HO8910 cells and promote cell migration and invasion in the recipient cells of ovarian cancer. And CD44 could be transferred to the HO8910 cells through exosomes from HO8910PM cells and influence the migration and invasion ability of HO8910 cells.
The more aggressive subpopulation can transfer a metastatic phenotype to the less one via secreting exosomes within a heterogeneous tumor. CD44 may be a potential therapeutic approach for ovarian cancer.
探讨肿瘤来源的外泌体在卵巢癌细胞体外进展和转移中的详细作用和机制。
采用差速离心法分离外泌体；透射电子显微镜、纳米 S90 和 Western blot 分别鉴定外泌体的形态、大小和生物标志物；Trans-well 室试验评估摄取 HO8910PM 细胞来源的外泌体的受体细胞的迁移和侵袭能力。通过质谱筛选下游分子。Western blot 鉴定 CD44，Trans-well 室试验和 CCK8 试验鉴定 CD44 的功能。
HO8910PM 细胞来源的外泌体可转移至 HO8910 细胞，并促进卵巢癌细胞中受体细胞的迁移和侵袭。并且 CD44 可以通过 HO8910PM 细胞来源的外泌体转移到 HO8910 细胞，并影响 HO8910 细胞的迁移和侵袭能力。
在异质性肿瘤中，侵袭性较强的亚群可以通过分泌外泌体将转移表型转移到侵袭性较弱的亚群。CD44 可能是治疗卵巢癌的潜在方法。
