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纳秒级脉冲电场对转谷氨酰胺酶2的钙依赖性激活作用。

Calcium-dependent activation of transglutaminase 2 by nanosecond pulsed electric fields.

作者信息

Morotomi-Yano Keiko, Yano Ken-Ichi

机构信息

Department of Bioelectrics Institute of Pulsed Power Science Kumamoto University Japan.

出版信息

FEBS Open Bio. 2017 Jun 9;7(7):934-943. doi: 10.1002/2211-5463.12227. eCollection 2017 Jul.

DOI:10.1002/2211-5463.12227
PMID:28680807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5494297/
Abstract

Exposure of cultured human cells to nanosecond pulsed electric fields (nsPEFs) elicits various cellular events, including Ca influx and cell death. Recently, nsPEFs have been regarded as a novel physical treatment useful for biology and medicine, but the underlying mechanism of action remains to be fully elucidated. In this study, we investigated the effect of nsPEFs on transglutaminases (TGs), enzymes that catalyze covalent protein modifications such as protein-protein crosslinking. Cellular TG activity was monitored by conjugation of cellular proteins with biotin-cadaverine, a cell-permeable pseudosubstrate for TGs. We applied nsPEFs to HeLa S3 cells and found that overall catalytic activity of cellular TGs was greatly increased in a Ca-dependent manner. The Ca ionophore ionomycin significantly augmented nsPEF-induced TG activation, further supporting the importance of Ca. Among human TG family members, TG2 is known to be the most ubiquitously expressed, and its catalytic activity requires elevated intracellular Ca. Given the requirement of Ca for TG activation by nsPEFs, we performed depletion of TG2 by RNA interference (RNAi). We observed that TG2 RNAi suppressed the nsPEF-induced TG activation and partially alleviated the cytotoxic effects of nsPEFs. These findings demonstrate that TG2 activation is a Ca-dependent event in nsPEF-exposed cells and exerts negative effects on cell physiology.

摘要

将培养的人类细胞暴露于纳秒级脉冲电场（nsPEFs）会引发各种细胞事件，包括钙离子内流和细胞死亡。最近，nsPEFs被视为一种对生物学和医学有用的新型物理治疗方法，但其潜在作用机制仍有待充分阐明。在本研究中，我们研究了nsPEFs对转谷氨酰胺酶（TGs）的影响，转谷氨酰胺酶是催化蛋白质共价修饰（如蛋白质-蛋白质交联）的酶。通过将细胞蛋白与生物素-尸胺（一种可渗透细胞的TGs假底物）结合来监测细胞TG活性。我们将nsPEFs应用于HeLa S3细胞，发现细胞TGs的总体催化活性以钙依赖的方式大幅增加。钙离子载体离子霉素显著增强了nsPEF诱导的TG激活，进一步支持了钙的重要性。在人类TG家族成员中，TG2是已知表达最广泛的，其催化活性需要细胞内钙离子浓度升高。鉴于nsPEFs激活TG需要钙离子，我们通过RNA干扰（RNAi）使TG2缺失。我们观察到TG2 RNAi抑制了nsPEF诱导的TG激活，并部分减轻了nsPEFs的细胞毒性作用。这些发现表明，TG2激活是nsPEF处理细胞中的一个钙依赖事件，并对细胞生理产生负面影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/668a76367ddd/FEB4-7-934-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/21ac093ea227/FEB4-7-934-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/a27963b2d37e/FEB4-7-934-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/881a625a4c40/FEB4-7-934-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/2635b9c131ee/FEB4-7-934-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/668a76367ddd/FEB4-7-934-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/21ac093ea227/FEB4-7-934-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/a27963b2d37e/FEB4-7-934-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/881a625a4c40/FEB4-7-934-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/2635b9c131ee/FEB4-7-934-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2768/5494297/668a76367ddd/FEB4-7-934-g005.jpg

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2
Gene electrotransfer clinical trials.基因电穿孔临床试验。
Adv Genet. 2015;89:235-262. doi: 10.1016/bs.adgen.2014.10.006. Epub 2014 Dec 4.
3
Calcium-mediated pore expansion and cell death following nanoelectroporation.纳米电穿孔后钙介导的孔扩张与细胞死亡。
纳秒级电脉冲诱导分化的 HL-60 细胞中外源释放染色体 DNA 和组蛋白瓜氨酸化。
Sci Rep. 2019 Jun 11;9(1):8451. doi: 10.1038/s41598-019-44817-9.
Biochim Biophys Acta. 2014 Oct;1838(10):2547-54. doi: 10.1016/j.bbamem.2014.06.015. Epub 2014 Jun 28.
4
Different involvement of extracellular calcium in two modes of cell death induced by nanosecond pulsed electric fields.细胞外钙在纳秒脉冲电场诱导的两种细胞死亡模式中的不同作用。
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5
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Cells. 2013 Mar 6;2(1):136-62. doi: 10.3390/cells2010136.
6
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7
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8
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9
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10
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